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Assay Detail

CHEMBL3855726

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human recombinant full length N-terminal His-tagged PI3Kgamma expressed in Sf9 insect cells using diC8PIP2 as substrate incubated for 15 mins followed by substrate addition measured after 2 hr by ADP-Glo luminescence assay
26
Total Activities
26
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type Sf9
Confidence 9 — Direct single protein target
Curated By Autocuration

Publication

Discovery of a Selective Phosphoinositide-3-Kinase (PI3K)-γ Inhibitor (IPI-549) as an Immuno-Oncology Clinical Candidate.
Evans CA, Liu T, Lescarbeau A, Nair SJ, Grenier L, Pradeilles JA, Glenadel Q, Tibbitts T, Rowley AM, DiNitto JP, Brophy EE, O'Hearn EL, Ali JA, Winkler DG, Goldstein SI, O'Hearn P, Martin CM, Hoyt JG, Soglia JR, Cheung C, Pink MM, Proctor JL, Palombella VJ, Tremblay MR, Castro AC.
ACS Med Chem Lett (2016) 7 : 862 -867
PubMed 27660692 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 26 6.80 7.85

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3975359 1 7.85
CHEMBL3984425 EGANELISIB 2.0 1 7.80
CHEMBL3937119 1 7.60
CHEMBL3963736 1 7.40
CHEMBL3976330 1 7.30
CHEMBL3911278 1 7.22
CHEMBL3923629 1 7.12
CHEMBL3914602 1 7.00
CHEMBL3928146 1 6.96
CHEMBL3921445 1 6.96
CHEMBL3947903 1 6.96
CHEMBL3959293 1 6.82
CHEMBL3893430 1 6.77
CHEMBL3944056 1 6.62
CHEMBL3966879 1 6.55
CHEMBL3917188 1 6.52
CHEMBL3946030 1 6.48
CHEMBL3955769 1 6.46
CHEMBL3895629 1 6.44
CHEMBL3908224 1 6.40
CHEMBL3916095 1 6.00
CHEMBL3978650 1 6.00
CHEMBL3983349 1 5.96
CHEMBL3954718 1 5.96
CHEMBL3890228 1 -
CHEMBL3986947 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3975359 IC50 = 14.0 nM 7.85
CHEMBL3984425 EGANELISIB IC50 = 16.0 nM 7.80
CHEMBL3937119 IC50 = 25.0 nM 7.60
CHEMBL3963736 IC50 = 40.0 nM 7.40
CHEMBL3976330 IC50 = 50.0 nM 7.30
CHEMBL3911278 IC50 = 60.0 nM 7.22
CHEMBL3923629 IC50 = 75.0 nM 7.12
CHEMBL3914602 IC50 = 100.0 nM 7.00
CHEMBL3928146 IC50 = 110.0 nM 6.96
CHEMBL3921445 IC50 = 110.0 nM 6.96
CHEMBL3947903 IC50 = 110.0 nM 6.96
CHEMBL3959293 IC50 = 150.0 nM 6.82
CHEMBL3893430 IC50 = 170.0 nM 6.77
CHEMBL3944056 IC50 = 240.0 nM 6.62
CHEMBL3966879 IC50 = 280.0 nM 6.55
CHEMBL3917188 IC50 = 300.0 nM 6.52
CHEMBL3946030 IC50 = 330.0 nM 6.48
CHEMBL3955769 IC50 = 350.0 nM 6.46
CHEMBL3895629 IC50 = 360.0 nM 6.44
CHEMBL3908224 IC50 = 400.0 nM 6.40
CHEMBL3916095 IC50 = 1000.0 nM 6.00
CHEMBL3978650 IC50 = 1000.0 nM 6.00
CHEMBL3983349 IC50 = 1100.0 nM 5.96
CHEMBL3954718 IC50 = 1100.0 nM 5.96
CHEMBL3890228 IC50 > 7000.0 nM -
CHEMBL3986947 IC50 > 10000.0 nM -