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Assay Detail

CHEMBL3858191

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of Pseudomonas aeruginosa DNA gyrase A assessed as inhibition of DNA supercoiling using PBR322 DNA as substrate after 30 mins by SYBR Gold staining based agarose gel electrophoresis
4
Total Activities
4
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Pseudomonas aeruginosa
Confidence 0 — Uncurated / Unknown
Curated By Autocuration

Target

Unchecked (CHEMBL612545)
Type UNCHECKED

Publication

Hybrid Antibiotic Overcomes Resistance in P. aeruginosa by Enhancing Outer Membrane Penetration and Reducing Efflux.
Gorityala BK, Guchhait G, Goswami S, Fernando DM, Kumar A, Zhanel GG, Schweizer F.
J Med Chem (2016) 59 : 8441 -8455
PubMed 27524179 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 4 5.28 5.68

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3960533 1 5.68
CHEMBL32 MOXIFLOXACIN 4.0 1 5.58
CHEMBL3899010 1 5.58
CHEMBL3955713 1 4.26

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3960533 IC50 = 2100.0 nM 5.68
CHEMBL32 MOXIFLOXACIN IC50 = 2600.0 nM 5.58
CHEMBL3899010 IC50 = 2600.0 nM 5.58
CHEMBL3955713 IC50 = 54700.0 nM 4.26