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Assay Detail

CHEMBL3858376

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Antagonist activity at recombinant human P2X7 receptor expressed in human 1321N1 cells assessed as inhibition of Bz-ATP-induced Ca2+ flux preincubated for 30 mins followed by agonist addition measured after 180 sec by calcium-4 dye based FLIPR assay
20
Total Activities
20
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type 1321-N1
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

P2X purinoceptor 7 (CHEMBL4805)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Identification of (R)-(2-Chloro-3-(trifluoromethyl)phenyl)(1-(5-fluoropyridin-2-yl)-4-methyl-6,7-dihydro-1H-imidazo[4,5-c]pyridin-5(4H)-yl)methanone (JNJ 54166060), a Small Molecule Antagonist of the P2X7 receptor.
Swanson DM, Savall BM, Coe KJ, Schoetens F, Koudriakova T, Skaptason J, Wall J, Rech J, Deng X, De Angelis M, Everson A, Lord B, Wang Q, Ao H, Scott B, Sepassi K, Lovenberg TW, Carruthers NI, Bhattacharya A, Letavic MA.
J Med Chem (2016) 59 : 8535 -8548
PubMed 27548392 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 20 7.48 8.40

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3895947 1 8.40
CHEMBL3906769 1 8.40
CHEMBL3901310 1 8.30
CHEMBL3947089 1 8.05
CHEMBL3913899 1 8.05
CHEMBL3911413 1 8.00
CHEMBL3956010 1 7.92
CHEMBL3962858 1 7.85
CHEMBL3955145 1 7.82
CHEMBL3919300 1 7.55
CHEMBL3910372 1 7.52
CHEMBL3960075 1 7.47
CHEMBL3901705 1 7.47
CHEMBL3904907 1 7.17
CHEMBL3898553 1 7.11
CHEMBL3914545 1 7.11
CHEMBL3907060 1 7.05
CHEMBL3948603 1 6.94
CHEMBL3894854 1 6.06
CHEMBL3929261 1 5.45

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3895947 IC50 = 4.0 nM 8.40
CHEMBL3906769 IC50 = 4.0 nM 8.40
CHEMBL3901310 IC50 = 5.0 nM 8.30
CHEMBL3947089 IC50 = 9.0 nM 8.05
CHEMBL3913899 IC50 = 9.0 nM 8.05
CHEMBL3911413 IC50 = 10.0 nM 8.00
CHEMBL3956010 IC50 = 12.0 nM 7.92
CHEMBL3962858 IC50 = 14.0 nM 7.85
CHEMBL3955145 IC50 = 15.0 nM 7.82
CHEMBL3919300 IC50 = 28.0 nM 7.55
CHEMBL3910372 IC50 = 30.0 nM 7.52
CHEMBL3960075 IC50 = 34.0 nM 7.47
CHEMBL3901705 IC50 = 34.0 nM 7.47
CHEMBL3904907 IC50 = 67.0 nM 7.17
CHEMBL3898553 IC50 = 77.0 nM 7.11
CHEMBL3914545 IC50 = 78.0 nM 7.11
CHEMBL3907060 IC50 = 89.0 nM 7.05
CHEMBL3948603 IC50 = 114.0 nM 6.94
CHEMBL3894854 IC50 = 880.0 nM 6.06
CHEMBL3929261 IC50 = 3538.0 nM 5.45