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Assay Detail

CHEMBL3858844

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Positive allosteric modulation of human mGlu2 receptor expressed in CHO cell membranes assessed as potentiation of glutamate-induced effect incubated for 30 mins prior to [35S]GTPgamma addition measured after 30 mins by scintillation counting method
21
Total Activities
21
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type CHO
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Metabotropic glutamate receptor 2 (CHEMBL5137)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of 8-Trifluoromethyl-3-cyclopropylmethyl-7-[(4-(2,4-difluorophenyl)-1-piperazinyl)methyl]-1,2,4-triazolo[4,3-a]pyridine (JNJ-46356479), a Selective and Orally Bioavailable mGlu2 Receptor Positive Allosteric Modulator (PAM).
Cid JM, Tresadern G, Vega JA, de Lucas AI, Del Cerro A, Matesanz E, Linares ML, García A, Iturrino L, Pérez-Benito L, Macdonald GJ, Oehlrich D, Lavreysen H, Peeters L, Ceusters M, Ahnaou A, Drinkenburg W, Mackie C, Somers M, Trabanco AA.
J Med Chem (2016) 59 : 8495 -8507
PubMed 27579727 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 21 7.48 8.59

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3901998 1 8.59
CHEMBL3910866 1 8.01
CHEMBL3947590 1 7.87
CHEMBL3938796 1 7.82
CHEMBL3919807 1 7.82
CHEMBL3957311 1 7.77
CHEMBL2179319 1 7.77
CHEMBL3947764 1 7.68
CHEMBL3985987 1 7.66
CHEMBL3981882 1 7.58
CHEMBL3928875 1 7.58
CHEMBL3892865 1 7.42
CHEMBL3956434 1 7.24
CHEMBL3963034 1 7.24
CHEMBL3929786 1 7.24
CHEMBL3929950 1 7.19
CHEMBL3926416 1 7.11
CHEMBL3940264 1 7.08
CHEMBL3919978 1 6.88
CHEMBL3337527 JNJ-40411813 2.0 1 6.83
CHEMBL3937907 1 6.71

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3901998 EC50 = 2.6 nM 8.59
CHEMBL3910866 EC50 = 9.7 nM 8.01
CHEMBL3947590 EC50 = 13.5 nM 7.87
CHEMBL3938796 EC50 = 15.0 nM 7.82
CHEMBL3919807 EC50 = 15.0 nM 7.82
CHEMBL3957311 EC50 = 17.0 nM 7.77
CHEMBL2179319 EC50 = 17.0 nM 7.77
CHEMBL3947764 EC50 = 21.0 nM 7.68
CHEMBL3985987 EC50 = 22.0 nM 7.66
CHEMBL3981882 EC50 = 26.0 nM 7.58
CHEMBL3928875 EC50 = 26.0 nM 7.58
CHEMBL3892865 EC50 = 38.0 nM 7.42
CHEMBL3956434 EC50 = 58.0 nM 7.24
CHEMBL3963034 EC50 = 57.0 nM 7.24
CHEMBL3929786 EC50 = 57.0 nM 7.24
CHEMBL3929950 EC50 = 65.0 nM 7.19
CHEMBL3926416 EC50 = 78.0 nM 7.11
CHEMBL3940264 EC50 = 83.0 nM 7.08
CHEMBL3919978 EC50 = 132.0 nM 6.88
CHEMBL3337527 JNJ-40411813 EC50 = 147.0 nM 6.83
CHEMBL3937907 EC50 = 195.0 nM 6.71