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Assay Detail

CHEMBL3859533

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human recombinant GST-fused SIRT1 expressed in Escherichia coli DH5-alpha using [3H]acetic acid labeled MPSDKTIGG as substrate after overnight incubation by liquid scintillation counting method
1
Total Activities
1
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

NAD-dependent protein deacetylase sirtuin-1 (CHEMBL4506)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Guttiferone A Aggregates Modulate Silent Information Regulator 1 (SIRT1) Activity.
Cottet K, Xu B, Coric P, Bouaziz S, Michel S, Vidal M, Lallemand MC, Broussy S.
J Med Chem (2016) 59 : 9560 -9566
PubMed 27669815 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 1 5.05 5.05

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL443681 OBLONGIFOLIN C 1 5.05

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL443681 OBLONGIFOLIN C IC50 = 9000.0 nM 5.05