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Assay Detail

CHEMBL3859871

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Activation of human recombinant AMPK alpha2/beta1/gamma1 expressed in African green monkey COS7 cells assessed as increase in biotinylated AAC (1 to 120 residues) peptide phosphorylation at Ser-79 residue measured after 60 mins by Alphascreen assay
1
Total Activities
1
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type COS-7
Confidence 7 — Homologous single protein target
Curated By Autocuration

Target

AMPK alpha2/beta1/gamma1 (CHEMBL3038455)
Type PROTEIN COMPLEX
Organism Homo sapiens

Publication

Recent progress in the identification of adenosine monophosphate-activated protein kinase (AMPK) activators.
Cameron KO, Kurumbail RG.
Bioorg Med Chem Lett (2016) 26 : 5139 -5148
PubMed 27727125 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 1 7.57 7.57

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3957286 1 7.57

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3957286 EC50 = 27.0 nM 7.57