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Assay Detail

CHEMBL3860169

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of microtubule-stimulated ATPase activity of N-terminal His6-tagged/SUMO-fused human MPP1 motor domain (57 to 491 residues) expressed in Escherichia coli BL21 CodonPlus by pyruvate kinase/lactate dehydrogenase-linked assay
5
Total Activities
3
Compounds Tested
2
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Kinesin-like protein KIF20B (CHEMBL2021752)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Depsidones from Lichens as Natural Product Inhibitors of M-Phase Phosphoprotein 1, a Human Kinesin Required for Cytokinesis.
Talapatra SK, Rath O, Clayton E, Tomasi S, Kozielski F.
J Nat Prod (2016) 79 : 1576 -1585
PubMed 27300079 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 3 4.93 5.24
Imax 2 - -

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL171637 PHYSODIC ACID 2 5.24
CHEMBL368810 2 4.61
CHEMBL220486 VARIOLARIC ACID 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL171637 PHYSODIC ACID IC50 = 5800.0 nM 5.24
CHEMBL368810 IC50 = 24400.0 nM 4.61
CHEMBL368810 Imax = 90.0 % -
CHEMBL171637 PHYSODIC ACID Imax = 90.0 % -
CHEMBL220486 VARIOLARIC ACID IC50 - -