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Assay Detail

CHEMBL3860933

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of synthetic recombinant wild type GCase enzyme velaglucerase alfa (unknown origin) at pH 5.9 preincubated for 5 mins followed by addition of 4-Methylumbelliferyl beta-D-glucopyranoside as substrate measured after 30 mins by fluorescence analysis
42
Total Activities
42
Compounds Tested
2
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Lysosomal acid glucosylceramidase (CHEMBL2179)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Design and Synthesis of Potent Quinazolines as Selective β-Glucocerebrosidase Modulators.
Zheng J, Chen L, Schwake M, Silverman RB, Krainc D.
J Med Chem (2016) 59 : 8508 -8520
PubMed 27598312 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 39 6.66 8.28
Inhibition 3 - -

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3976182 1 8.28
CHEMBL3939100 1 8.19
CHEMBL3930102 1 8.09
CHEMBL3923120 1 8.08
CHEMBL3950881 1 8.06
CHEMBL3923147 1 8.00
CHEMBL3976949 1 7.90
CHEMBL3956777 1 7.70
CHEMBL3956552 1 7.68
CHEMBL3964444 1 7.57
CHEMBL3968646 1 7.53
CHEMBL3941104 1 7.46
CHEMBL3974789 1 7.38
CHEMBL3948124 1 7.25
CHEMBL1193417 1 7.01
CHEMBL3906252 1 6.91
CHEMBL3929224 1 6.90
CHEMBL3957610 1 6.78
CHEMBL3920301 1 6.75
CHEMBL3980248 1 6.63
CHEMBL3965871 1 6.60
CHEMBL3970989 1 6.55
CHEMBL3963682 1 6.39
CHEMBL3894465 1 6.37
CHEMBL3968279 1 6.14
CHEMBL3970658 1 6.09
CHEMBL3898722 1 6.07
CHEMBL3932095 1 6.06
CHEMBL3921895 1 6.05
CHEMBL3908863 1 6.03

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3976182 IC50 = 5.2 nM 8.28
CHEMBL3939100 IC50 = 6.5 nM 8.19
CHEMBL3930102 IC50 = 8.2 nM 8.09
CHEMBL3923120 IC50 = 8.3 nM 8.08
CHEMBL3950881 IC50 = 8.7 nM 8.06
CHEMBL3923147 IC50 = 9.9 nM 8.00
CHEMBL3976949 IC50 = 12.5 nM 7.90
CHEMBL3956777 IC50 = 20.0 nM 7.70
CHEMBL3956552 IC50 = 21.1 nM 7.68
CHEMBL3964444 IC50 = 27.0 nM 7.57
CHEMBL3968646 IC50 = 29.4 nM 7.53
CHEMBL3941104 IC50 = 35.0 nM 7.46
CHEMBL3974789 IC50 = 42.0 nM 7.38
CHEMBL3948124 IC50 = 56.0 nM 7.25
CHEMBL1193417 IC50 = 97.0 nM 7.01
CHEMBL3906252 IC50 = 122.0 nM 6.91
CHEMBL3929224 IC50 = 126.0 nM 6.90
CHEMBL3957610 IC50 = 168.0 nM 6.78
CHEMBL3920301 IC50 = 177.0 nM 6.75
CHEMBL3980248 IC50 = 234.0 nM 6.63
CHEMBL3965871 IC50 = 251.0 nM 6.60
CHEMBL3970989 IC50 = 282.0 nM 6.55
CHEMBL3963682 IC50 = 405.0 nM 6.39
CHEMBL3894465 IC50 = 431.0 nM 6.37
CHEMBL3968279 IC50 = 720.0 nM 6.14
CHEMBL3970658 IC50 = 821.0 nM 6.09
CHEMBL3898722 IC50 = 857.0 nM 6.07
CHEMBL3932095 IC50 = 872.0 nM 6.06
CHEMBL3921895 IC50 = 891.0 nM 6.05
CHEMBL3908863 IC50 = 926.0 nM 6.03
CHEMBL3972062 IC50 = 1280.0 nM 5.89
CHEMBL3913022 IC50 = 1400.0 nM 5.85
CHEMBL3942452 IC50 = 1530.0 nM 5.82
CHEMBL3944808 IC50 = 2840.0 nM 5.55
CHEMBL3914158 IC50 = 4290.0 nM 5.37
CHEMBL119841 IC50 = 5770.0 nM 5.24
CHEMBL3979185 IC50 = 28070.0 nM 4.55
CHEMBL3912910 IC50 = 33210.0 nM 4.48
CHEMBL1405704 IC50 = 36070.0 nM 4.44
CHEMBL3902851 Inhibition - % -
CHEMBL3893897 Inhibition - % -
CHEMBL1591893 Inhibition - % -