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Assay Detail

CHEMBL3861027

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of recombinant N-terminal GST tagged PDE6C (unknown origin) expressed in baculovirus infected Sf9 cells using cGMP as substrate by malachite green reagent based assay
2
Total Activities
2
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type Sf9
Confidence 9 — Direct single protein target
Curated By Autocuration

Publication

Design, Synthesis, and Biological Evaluation of First-in-Class Dual Acting Histone Deacetylases (HDACs) and Phosphodiesterase 5 (PDE5) Inhibitors for the Treatment of Alzheimer's Disease.
Rabal O, Sánchez-Arias JA, Cuadrado-Tejedor M, de Miguel I, Pérez-González M, García-Barroso C, Ugarte A, Estella-Hermoso de Mendoza A, Sáez E, Espelosin M, Ursua S, Haizhong T, Wei W, Musheng X, Garcia-Osta A, Oyarzabal J.
J Med Chem (2016) 59 : 8967 -9004
PubMed 27606546 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 2 9.24 9.89

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3936651 1 9.89
CHEMBL3900593 1 8.59

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3936651 IC50 = 0.13 nM 9.89
CHEMBL3900593 IC50 = 2.6 nM 8.59