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Assay Detail

CHEMBL3862736

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of His-tagged human Aurora A kinase (122 to 40 residues) expressed in Escherichia coli BL21 (DE3) Rosetta cells using biotinylated STK2 substrate incubated for 30 mins in presence of 0.002 uM TPX2 by HTRF assay
1
Total Activities
1
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Aurora kinase A (CHEMBL4722)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of a Selective Aurora A Kinase Inhibitor by Virtual Screening.
Kilchmann F, Marcaida MJ, Kotak S, Schick T, Boss SD, Awale M, Gönczy P, Reymond JL.
J Med Chem (2016) 59 : 7188 -7211
PubMed 27391133 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 1 8.77 8.77

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3921246 1 8.77

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3921246 IC50 = 1.7 nM 8.77