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Assay Detail

CHEMBL3864979

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human recombinant DTT-activated caspase-5 expressed in Escherichia coli using Ac-WEHD-AMC as substrate preincubated for 30 mins followed by substrate addition by fluorescence assay
30
Total Activities
15
Compounds Tested
2
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Caspase-5 (CHEMBL3131)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Variation of the aryl substituent on the piperazine ring within the 4-(piperazin-1-yl)-2,6-di(pyrrolidin-1-yl)pyrimidine scaffold unveils potent, non-competitive inhibitors of the inflammatory caspases.
Kent CR, Bryja M, Gustafson HA, Kawarski MY, Lenti G, Pierce EN, Knopp RC, Ceja V, Pati B, Walters DE, Karver CE.
Bioorg Med Chem Lett (2016) 26 : 5476 -5480
PubMed 27777011 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 15 6.63 7.15
Ki 15 7.27 7.80

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3949842 2 7.80
CHEMBL3904435 2 7.62
CHEMBL3970645 2 7.55
CHEMBL3965572 2 7.47
CHEMBL3941088 2 7.47
CHEMBL3932441 2 7.39
CHEMBL3905486 2 7.33
CHEMBL3898411 2 7.32
CHEMBL3895496 2 7.23
CHEMBL3978901 2 7.23
CHEMBL3898379 2 7.09
CHEMBL3984293 2 7.00
CHEMBL3939229 2 6.96
CHEMBL3968765 2 6.87
CHEMBL1412861 2 6.68

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3949842 Ki = 16.0 nM 7.80
CHEMBL3904435 Ki = 24.0 nM 7.62
CHEMBL3970645 Ki = 28.0 nM 7.55
CHEMBL3941088 Ki = 34.0 nM 7.47
CHEMBL3965572 Ki = 34.0 nM 7.47
CHEMBL3932441 Ki = 41.0 nM 7.39
CHEMBL3905486 Ki = 47.0 nM 7.33
CHEMBL3898411 Ki = 48.0 nM 7.32
CHEMBL3978901 Ki = 59.0 nM 7.23
CHEMBL3895496 Ki = 59.0 nM 7.23
CHEMBL3949842 IC50 = 71.0 nM 7.15
CHEMBL3898379 Ki = 81.0 nM 7.09
CHEMBL3984293 Ki = 99.0 nM 7.00
CHEMBL3904435 IC50 = 105.0 nM 6.98
CHEMBL3939229 Ki = 111.0 nM 6.96
CHEMBL3970645 IC50 = 123.0 nM 6.91
CHEMBL3968765 Ki = 135.0 nM 6.87
CHEMBL3965572 IC50 = 146.0 nM 6.84
CHEMBL3941088 IC50 = 149.0 nM 6.83
CHEMBL3932441 IC50 = 178.0 nM 6.75
CHEMBL3905486 IC50 = 205.0 nM 6.69
CHEMBL1412861 Ki = 210.0 nM 6.68
CHEMBL3898411 IC50 = 210.0 nM 6.68
CHEMBL3895496 IC50 = 254.0 nM 6.59
CHEMBL3978901 IC50 = 254.0 nM 6.59
CHEMBL3898379 IC50 = 350.0 nM 6.46
CHEMBL3984293 IC50 = 430.0 nM 6.37
CHEMBL3939229 IC50 = 482.0 nM 6.32
CHEMBL3968765 IC50 = 586.0 nM 6.23
CHEMBL1412861 IC50 = 910.0 nM 6.04