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Assay Detail

CHEMBL3865469

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Binding affinity to human His6-tagged SETD8 (192 to 352 residues) C302S mutant in PBS buffer in presence of SAM by ITC assay
15
Total Activities
15
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 0 — Uncurated / Unknown
Curated By Autocuration

Target

Unchecked (CHEMBL612545)
Type UNCHECKED

Publication

Turning a Substrate Peptide into a Potent Inhibitor for the Histone Methyltransferase SETD8.
Judge RA, Zhu H, Upadhyay AK, Bodelle PM, Hutchins CW, Torrent M, Marin VL, Yu W, Vedadi M, Li F, Brown PJ, Pappano WN, Sun C, Petros AM.
ACS Med Chem Lett (2016) 7 : 1102 -1106
PubMed 27994746 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Kd 15 5.94 6.92

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3979628 1 6.92
CHEMBL3934996 1 6.85
CHEMBL3909400 1 6.68
CHEMBL3894573 1 6.50
CHEMBL3961424 1 6.46
CHEMBL3918322 1 6.24
CHEMBL3960411 1 6.10
CHEMBL3986240 1 5.74
CHEMBL3957541 1 5.52
CHEMBL3891356 1 5.33
CHEMBL3933297 1 5.25
CHEMBL3905280 1 4.83
CHEMBL3933942 1 4.82
CHEMBL3954512 1 -
CHEMBL3971503 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3979628 Kd = 120.0 nM 6.92
CHEMBL3934996 Kd = 140.0 nM 6.85
CHEMBL3909400 Kd = 210.0 nM 6.68
CHEMBL3894573 Kd = 320.0 nM 6.50
CHEMBL3961424 Kd = 350.0 nM 6.46
CHEMBL3918322 Kd = 580.0 nM 6.24
CHEMBL3960411 Kd = 800.0 nM 6.10
CHEMBL3986240 Kd = 1840.0 nM 5.74
CHEMBL3957541 Kd = 3040.0 nM 5.52
CHEMBL3891356 Kd = 4640.0 nM 5.33
CHEMBL3933297 Kd = 5570.0 nM 5.25
CHEMBL3905280 Kd = 14800.0 nM 4.83
CHEMBL3933942 Kd = 15000.0 nM 4.82
CHEMBL3954512 Kd - -
CHEMBL3971503 Kd - -