Skip to main content
Free research Free research Free assay review with research home and workspace preview
Quick search ChEMBL 36
Assay Detail

CHEMBL3865725

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of FAM-Bid binding to human BCL2 expressed in Escherichia coli BL21 after 30 mins by fluorescence polarization assay
24
Total Activities
10
Compounds Tested
2
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Apoptosis regulator Bcl-2 (CHEMBL4860)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Gossypol with Hydrophobic Linear Esters Exhibits Enhanced Antitumor Activity as an Inhibitor of Antiapoptotic Proteins.
Lu Y, Wu S, Yue Y, He S, Li J, Tang J, Wang W, Zhou HB.
ACS Med Chem Lett (2016) 7 : 1185 -1190
PubMed 27994761 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 12 6.32 6.99
Ki 12 6.82 8.30

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3929718 2 8.30
CHEMBL3957283 2 7.05
CHEMBL3918448 6 6.88
CHEMBL1962393 2 6.77
CHEMBL3932954 2 6.74
CHEMBL1962390 2 6.65
CHEMBL3951720 2 6.63
CHEMBL3971530 2 6.46
CHEMBL51483 GOSSYPOL 3.0 2 6.29
CHEMBL3137309 VENETOCLAX 4.0 2 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3929718 Ki = 5.0 nM 8.30
CHEMBL3957283 Ki = 90.0 nM 7.05
CHEMBL3929718 IC50 = 103.0 nM 6.99
CHEMBL3918448 Ki = 131.0 nM 6.88
CHEMBL3918448 Ki = 167.0 nM 6.78
CHEMBL1962393 Ki = 171.0 nM 6.77
CHEMBL3932954 Ki = 182.0 nM 6.74
CHEMBL1962390 Ki = 224.0 nM 6.65
CHEMBL3951720 Ki = 235.0 nM 6.63
CHEMBL3957283 IC50 = 286.0 nM 6.54
CHEMBL3918448 Ki = 341.0 nM 6.47
CHEMBL3971530 Ki = 348.0 nM 6.46
CHEMBL3918448 IC50 = 375.0 nM 6.43
CHEMBL1962393 IC50 = 458.0 nM 6.34
CHEMBL3918448 IC50 = 452.0 nM 6.34
CHEMBL3932954 IC50 = 483.0 nM 6.32
CHEMBL51483 GOSSYPOL Ki = 511.0 nM 6.29
CHEMBL1962390 IC50 = 573.0 nM 6.24
CHEMBL3951720 IC50 = 596.0 nM 6.22
CHEMBL3971530 IC50 = 840.0 nM 6.08
CHEMBL3918448 IC50 = 825.0 nM 6.08
CHEMBL51483 GOSSYPOL IC50 = 1191.0 nM 5.92
CHEMBL3137309 VENETOCLAX IC50 < 1.0 nM -
CHEMBL3137309 VENETOCLAX Ki < 1.0 nM -