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Assay Detail

CHEMBL3867232

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of NAMPT in human HT1080 cells assessed as decrease in cell viability by measuring plasma protein binding corrected IC50 after 96 hrs by CyQuant-Direct reagent based assay
1
Total Activities
1
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Tissue Plasma
Cell Type HT-1080
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Nicotinamide phosphoribosyltransferase (CHEMBL1744525)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Minimizing CYP2C9 Inhibition of Exposed-Pyridine NAMPT (Nicotinamide Phosphoribosyltransferase) Inhibitors.
Zak M, Yuen PW, Liu X, Patel S, Sampath D, Oeh J, Liederer BM, Wang W, O'Brien T, Xiao Y, Skelton N, Hua R, Sodhi J, Wang Y, Zhang L, Zhao G, Zheng X, Ho YC, Bair KW, Dragovich PS.
J Med Chem (2016) 59 : 8345 -8368
PubMed 27541271 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 1 8.49 8.49

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3916438 1 8.49

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3916438 IC50 = 3.2 nM 8.49