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Assay Detail

CHEMBL3867797

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human ABCG2 R482 mutant transfected in HEK293 cells assessed as potentiation of mitoxantrone-induced cytotoxicity by measuring mitoxantrone IC50 at 3000 nM after 72 hrs by CCK8 assay (Rvb = 107.40 +/- 13.93 nM)
1
Total Activities
1
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK293
Confidence 0 — Uncurated / Unknown
Curated By Autocuration

Target

Unchecked (CHEMBL612545)
Type UNCHECKED

Publication

Hernandezine, a Bisbenzylisoquinoline Alkaloid with Selective Inhibitory Activity against Multidrug-Resistance-Linked ATP-Binding Cassette Drug Transporter ABCB1.
Hsiao SH, Lu YJ, Yang CC, Tuo WC, Li YQ, Huang YH, Hsieh CH, Hung TH, Wu CP.
J Nat Prod (2016) 79 : 2135 -2142
PubMed 27504669 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 1 8.27 8.27

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL410316 FUMITREMORGIN 1 8.27

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL410316 FUMITREMORGIN IC50 = 5.42 nM 8.27