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Assay Detail

CHEMBL3868063

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of GST-tagged recombinant human JAK2 expressed in baculovirus using TK-substrate-biotin as substrate preincubated for 5 mins followed by substrate addition after 30 mins by HTRF assay
18
Total Activities
18
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Tyrosine-protein kinase JAK2 (CHEMBL2971)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Identification of 4-(2-furanyl)pyrimidin-2-amines as Janus kinase 2 inhibitors.
Wang Y, Huang W, Xin M, Chen P, Gui L, Zhao X, Tang F, Wang J, Liu F.
Bioorg Med Chem (2017) 25 : 75 -83
PubMed 27771180 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 18 7.44 9.15

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3940537 1 9.15
CHEMBL221959 TOFACITINIB 4.0 1 8.55
CHEMBL3907087 1 8.05
CHEMBL3970092 1 8.03
CHEMBL3966342 1 7.98
CHEMBL3948663 1 7.88
CHEMBL3912973 1 7.78
CHEMBL3895231 1 7.72
CHEMBL3903955 1 7.59
CHEMBL3950301 1 7.42
CHEMBL3920857 1 7.37
CHEMBL3922588 1 7.27
CHEMBL3913592 1 7.26
CHEMBL3895028 1 6.86
CHEMBL3941571 1 6.79
CHEMBL3974845 1 6.65
CHEMBL3934182 1 6.49
CHEMBL3955194 1 5.15

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3940537 IC50 = 0.7 nM 9.15
CHEMBL221959 TOFACITINIB IC50 = 2.8 nM 8.55
CHEMBL3907087 IC50 = 8.9 nM 8.05
CHEMBL3970092 IC50 = 9.4 nM 8.03
CHEMBL3966342 IC50 = 10.4 nM 7.98
CHEMBL3948663 IC50 = 13.2 nM 7.88
CHEMBL3912973 IC50 = 16.6 nM 7.78
CHEMBL3895231 IC50 = 18.9 nM 7.72
CHEMBL3903955 IC50 = 25.7 nM 7.59
CHEMBL3950301 IC50 = 37.9 nM 7.42
CHEMBL3920857 IC50 = 42.9 nM 7.37
CHEMBL3922588 IC50 = 53.7 nM 7.27
CHEMBL3913592 IC50 = 55.4 nM 7.26
CHEMBL3895028 IC50 = 136.7 nM 6.86
CHEMBL3941571 IC50 = 164.0 nM 6.79
CHEMBL3974845 IC50 = 226.1 nM 6.65
CHEMBL3934182 IC50 = 324.5 nM 6.49
CHEMBL3955194 IC50 = 7089.0 nM 5.15