Assay Detail
Binding
CHEMBL3868074
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of recombinant CYP21 (unknown origin) overexpressed in human AD293 cells assessed as reduction in 11-deoxycortisol formation preincubated for 60 mins followed by addition of 17-alpha-hydroxyprogesterone as substrate measured after 45 mins by LC-MS/MS analysis
13
Total Activities
13
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Cell Type | AD293 |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Publication
Design, synthesis, and evaluation of (2S,4R)-Ketoconazole sulfonamide analogs as potential treatments for Metabolic Syndrome.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 13 | 6.99 | 8.10 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL3891718 | — | — | 1 | 8.10 |
| CHEMBL3980889 | — | — | 1 | 7.70 |
| CHEMBL3968178 | — | — | 1 | 7.58 |
| CHEMBL3922888 | — | — | 1 | 7.55 |
| CHEMBL3938615 | — | — | 1 | 7.31 |
| CHEMBL3894912 | — | — | 1 | 7.29 |
| CHEMBL3952032 | — | — | 1 | 7.11 |
| CHEMBL3931836 | — | — | 1 | 6.91 |
| CHEMBL3960724 | — | — | 1 | 6.83 |
| CHEMBL3964421 | — | — | 1 | 6.78 |
| CHEMBL3913908 | — | — | 1 | 6.57 |
| CHEMBL3983734 | — | — | 1 | 5.80 |
| CHEMBL157101 | KETOCONAZOLE | 4.0 | 1 | 5.37 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL3891718 | — | IC50 | = | 8.0 | nM | 8.10 |
| CHEMBL3980889 | — | IC50 | = | 20.0 | nM | 7.70 |
| CHEMBL3968178 | — | IC50 | = | 26.0 | nM | 7.58 |
| CHEMBL3922888 | — | IC50 | = | 28.0 | nM | 7.55 |
| CHEMBL3938615 | — | IC50 | = | 49.0 | nM | 7.31 |
| CHEMBL3894912 | — | IC50 | = | 51.0 | nM | 7.29 |
| CHEMBL3952032 | — | IC50 | = | 78.0 | nM | 7.11 |
| CHEMBL3931836 | — | IC50 | = | 123.0 | nM | 6.91 |
| CHEMBL3960724 | — | IC50 | = | 148.0 | nM | 6.83 |
| CHEMBL3964421 | — | IC50 | = | 166.0 | nM | 6.78 |
| CHEMBL3913908 | — | IC50 | = | 271.0 | nM | 6.57 |
| CHEMBL3983734 | — | IC50 | = | 1600.0 | nM | 5.80 |
| CHEMBL157101 | KETOCONAZOLE | IC50 | = | 4300.0 | nM | 5.37 |