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Assay Detail

CHEMBL3869409

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human lysosomal beta-glucosidase using 4-methylumbelliferyl-beta-D-glucopyranoside as substrate preincubated for 45 mins followed by substrate addition measured after 30 mins by fluorescence spectrophotometric method
10
Total Activities
10
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 0 — Uncurated / Unknown
Curated By Autocuration

Target

Unchecked (CHEMBL612545)
Type UNCHECKED

Publication

Strategy for designing selective α-l-rhamnosidase inhibitors: Synthesis and biological evaluation of l-DMDP cyclic isothioureas.
Miyawaki S, Hirokami Y, Kinami K, Hoshino M, Minehira D, Miyamoto D, Nash RJ, Fleet GW, Adachi I, Toyooka N, Kato A.
Bioorg Med Chem (2017) 25 : 107 -115
PubMed 27789075 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 10 5.40 6.80

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3935498 1 6.80
CHEMBL3907555 1 6.13
CHEMBL3897971 1 6.00
CHEMBL3901083 1 6.00
CHEMBL3939205 1 5.62
CHEMBL3944457 1 4.92
CHEMBL3916914 1 4.80
CHEMBL3970812 1 4.19
CHEMBL3891002 1 4.14
CHEMBL3943978 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3935498 IC50 = 160.0 nM 6.80
CHEMBL3907555 IC50 = 740.0 nM 6.13
CHEMBL3897971 IC50 = 1000.0 nM 6.00
CHEMBL3901083 IC50 = 1000.0 nM 6.00
CHEMBL3939205 IC50 = 2400.0 nM 5.62
CHEMBL3944457 IC50 = 12000.0 nM 4.92
CHEMBL3916914 IC50 = 16000.0 nM 4.80
CHEMBL3970812 IC50 = 65000.0 nM 4.19
CHEMBL3891002 IC50 = 73000.0 nM 4.14
CHEMBL3943978 IC50 = 532000.0 nM -