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Assay Detail

CHEMBL3870929

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of recombinant GST-His-tagged Keap1-DC domain (321 to 609 residues) (unknown origin) expressed in Escherichia coli/FAM-labeled Nrf2 (unknown origin) interaction measured after 30 mins by fluorescence polarization assay
10
Total Activities
10
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 5 — Multiple protein complex / RNA
Curated By Autocuration

Target

Keap1/Nrf2 (CHEMBL3038498)
Type PROTEIN-PROTEIN INTERACTION
Organism Homo sapiens

Publication

Synthesis of Keap1-phosphorylated p62 and Keap1-Nrf2 protein-protein interaction inhibitors and their inhibitory activity.
Yasuda D, Nakajima M, Yuasa A, Obata R, Takahashi K, Ohe T, Ichimura Y, Komatsu M, Yamamoto M, Imamura R, Kojima H, Okabe T, Nagano T, Mashino T.
Bioorg Med Chem Lett (2016) 26 : 5956 -5959
PubMed 27839920 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 10 6.07 6.82

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3962296 1 6.82
CHEMBL3925906 1 6.70
CHEMBL3928944 1 6.64
CHEMBL3906697 1 6.50
CHEMBL3953535 1 6.14
CHEMBL3934789 1 5.89
CHEMBL2402207 1 5.75
CHEMBL3939457 1 5.55
CHEMBL3977467 1 5.50
CHEMBL3948237 1 5.21

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3962296 IC50 = 150.0 nM 6.82
CHEMBL3925906 IC50 = 200.0 nM 6.70
CHEMBL3928944 IC50 = 230.0 nM 6.64
CHEMBL3906697 IC50 = 320.0 nM 6.50
CHEMBL3953535 IC50 = 720.0 nM 6.14
CHEMBL3934789 IC50 = 1300.0 nM 5.89
CHEMBL2402207 IC50 = 1800.0 nM 5.75
CHEMBL3939457 IC50 = 2800.0 nM 5.55
CHEMBL3977467 IC50 = 3200.0 nM 5.50
CHEMBL3948237 IC50 = 6200.0 nM 5.21