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Assay Detail

CHEMBL3870971

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Antagonist activity at human Histamine H1 receptor expressed in CHO cells assessed as inhibition of histamine-induced calcium flux at 100 nM preincubated for 30 mins followed by histamine addition by Fluo-4-AM dye based FLIPR assay
7
Total Activities
7
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type CHO
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Histamine H1 receptor (CHEMBL231)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

The discovery of quinoline based single-ligand human H1 and H3 receptor antagonists.
Procopiou PA, Ancliff RA, Gore PM, Hancock AP, Hodgson ST, Holmes DS, Keeling SP, Looker BE, Parr NA, Rowedder JE, Slack RJ.
Bioorg Med Chem Lett (2016) 26 : 5855 -5859
PubMed 27866818 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Kd 7 8.67 9.70

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL639 AZELASTINE 4.0 1 9.70
CHEMBL1767164 GSK-1004723 2.0 1 9.10
CHEMBL3898341 1 8.80
CHEMBL3926412 1 8.60
CHEMBL3893197 1 8.30
CHEMBL3917428 1 8.20
CHEMBL3947980 1 8.00

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL639 AZELASTINE Kd = 0.1995 nM 9.70
CHEMBL1767164 GSK-1004723 Kd = 0.7943 nM 9.10
CHEMBL3898341 Kd = 1.585 nM 8.80
CHEMBL3926412 Kd = 2.512 nM 8.60
CHEMBL3893197 Kd = 5.012 nM 8.30
CHEMBL3917428 Kd = 6.31 nM 8.20
CHEMBL3947980 Kd = 10.0 nM 8.00