Assay Detail
Binding
CHEMBL3871206
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of recombinant human MIF tautomerase activity expressed in Escherichia coli assessed as borate-enol complex formation using 4-hydroxyphenyl pyruvic acid as substrate preincubated for 20 mins followed by substrate addition measured for 175 sec by fluorescence based analysis
18
Total Activities
18
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Target
Macrophage migration inhibitory factor (CHEMBL2085) ↗| Type | SINGLE PROTEIN |
| Organism | Homo sapiens |
Publication
Systematic Study of Effects of Structural Modifications on the Aqueous Solubility of Drug-like Molecules.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| Ki | 18 | 6.75 | 7.85 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL3917143 | — | — | 1 | 7.85 |
| CHEMBL3918231 | — | — | 1 | 7.62 |
| CHEMBL3890541 | — | — | 1 | 7.43 |
| CHEMBL3927658 | — | — | 1 | 7.41 |
| CHEMBL3899123 | — | — | 1 | 7.32 |
| CHEMBL3957289 | — | — | 1 | 7.13 |
| CHEMBL3960793 | — | — | 1 | 6.84 |
| CHEMBL3906441 | — | — | 1 | 6.83 |
| CHEMBL3798384 | — | — | 1 | 6.79 |
| CHEMBL3798149 | — | — | 1 | 6.70 |
| CHEMBL3924400 | — | — | 1 | 6.70 |
| CHEMBL3953102 | — | — | 1 | 6.64 |
| CHEMBL3897449 | — | — | 1 | 6.58 |
| CHEMBL3943378 | — | — | 1 | 6.57 |
| CHEMBL3915405 | — | — | 1 | 6.44 |
| CHEMBL3925522 | — | — | 1 | 6.28 |
| CHEMBL3945050 | — | — | 1 | 5.86 |
| CHEMBL3798703 | — | — | 1 | 4.56 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL3917143 | — | Ki | = | 14.0 | nM | 7.85 |
| CHEMBL3918231 | — | Ki | = | 24.0 | nM | 7.62 |
| CHEMBL3890541 | — | Ki | = | 37.0 | nM | 7.43 |
| CHEMBL3927658 | — | Ki | = | 39.0 | nM | 7.41 |
| CHEMBL3899123 | — | Ki | = | 48.0 | nM | 7.32 |
| CHEMBL3957289 | — | Ki | = | 74.0 | nM | 7.13 |
| CHEMBL3960793 | — | Ki | = | 144.0 | nM | 6.84 |
| CHEMBL3906441 | — | Ki | = | 147.0 | nM | 6.83 |
| CHEMBL3798384 | — | Ki | = | 161.0 | nM | 6.79 |
| CHEMBL3798149 | — | Ki | = | 200.0 | nM | 6.70 |
| CHEMBL3924400 | — | Ki | = | 200.0 | nM | 6.70 |
| CHEMBL3953102 | — | Ki | = | 230.0 | nM | 6.64 |
| CHEMBL3897449 | — | Ki | = | 260.0 | nM | 6.58 |
| CHEMBL3943378 | — | Ki | = | 270.0 | nM | 6.57 |
| CHEMBL3915405 | — | Ki | = | 360.0 | nM | 6.44 |
| CHEMBL3925522 | — | Ki | = | 530.0 | nM | 6.28 |
| CHEMBL3945050 | — | Ki | = | 1370.0 | nM | 5.86 |
| CHEMBL3798703 | — | Ki | = | 27300.0 | nM | 4.56 |