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Assay Detail

CHEMBL3872973

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of Leishmania major recombinant N-terminal hexa-His tagged PTR1 catalytic activity expressed in Escherichia coli BL21(DE3) using biopterin as substrate measured for 60 sec in presence of NADPH by UV-visible spectrophotometric method relative to control
3
Total Activities
3
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Leishmania major
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Pteridine reductase 1 (CHEMBL6194)
Type SINGLE PROTEIN
Organism Leishmania major

Publication

Structure-guided discovery of thiazolidine-2,4-dione derivatives as a novel class of Leishmania major pteridine reductase 1 inhibitors.
Leite FHA, Santiago PBGDS, Froes TQ, da Silva Filho J, da Silva SG, Ximenes RM, de Faria AR, Brondani DJ, de Albuquerque JFC, Castilho MS.
Eur J Med Chem (2016) 123 : 639 -648
PubMed 27517809 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 3 4.24 4.35

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3905744 1 4.35
CHEMBL3933757 1 4.12
CHEMBL3942723 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3905744 IC50 = 44670.0 nM 4.35
CHEMBL3933757 IC50 = 75860.0 nM 4.12
CHEMBL3942723 IC50 = 588840.0 nM -