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Assay Detail

CHEMBL3873939

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of wild-type HCV genotype 1b Con1 C-terminal truncated NS5B RNA dependent RNA polymerase expressed in Escherichia coli BL21(DE3) Star assessed as inhibition of [alpha-32P]UTP incorporation using polyrA/T12 as template/primer measured after 60 mins by TopCount NXT method
20
Total Activities
20
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Hepatitis C virus (isolate Con1)
Confidence 0 — Uncurated / Unknown
Curated By Autocuration

Target

Unchecked (CHEMBL612545)
Type UNCHECKED

Publication

Discovery and initial optimization of alkoxyanthranilic acid derivatives as inhibitors of HCV NS5B polymerase.
Parcella K, Nickel A, Beno BR, Sheriff S, Wan C, Wang YK, Roberts SB, Meanwell NA, Kadow JF.
Bioorg Med Chem Lett (2017) 27 : 295 -298
PubMed 27908764 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 20 5.19 6.06

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3940281 1 6.06
CHEMBL3957330 1 5.92
CHEMBL3921421 1 5.77
CHEMBL3929755 1 5.68
CHEMBL3902898 1 5.36
CHEMBL3920853 1 5.34
CHEMBL3983325 1 5.21
CHEMBL3930888 1 5.18
CHEMBL3902813 1 5.08
CHEMBL3982375 1 5.08
CHEMBL3947576 1 5.06
CHEMBL3903438 1 5.00
CHEMBL3893403 1 4.96
CHEMBL3892823 1 4.82
CHEMBL3974374 1 4.62
CHEMBL3902396 1 4.60
CHEMBL3948659 1 4.50
CHEMBL3930375 1 -
CHEMBL3912461 1 -
CHEMBL3976844 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3940281 IC50 = 880.0 nM 6.06
CHEMBL3957330 IC50 = 1200.0 nM 5.92
CHEMBL3921421 IC50 = 1700.0 nM 5.77
CHEMBL3929755 IC50 = 2100.0 nM 5.68
CHEMBL3902898 IC50 = 4400.0 nM 5.36
CHEMBL3920853 IC50 = 4600.0 nM 5.34
CHEMBL3983325 IC50 = 6200.0 nM 5.21
CHEMBL3930888 IC50 = 6600.0 nM 5.18
CHEMBL3902813 IC50 = 8400.0 nM 5.08
CHEMBL3982375 IC50 = 8300.0 nM 5.08
CHEMBL3947576 IC50 = 8700.0 nM 5.06
CHEMBL3903438 IC50 = 10000.0 nM 5.00
CHEMBL3893403 IC50 = 11000.0 nM 4.96
CHEMBL3892823 IC50 = 15000.0 nM 4.82
CHEMBL3974374 IC50 = 24000.0 nM 4.62
CHEMBL3902396 IC50 = 25000.0 nM 4.60
CHEMBL3948659 IC50 = 32000.0 nM 4.50
CHEMBL3930375 IC50 > 50000.0 nM -
CHEMBL3912461 IC50 > 50000.0 nM -
CHEMBL3976844 IC50 > 50000.0 nM -