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Assay Detail

CHEMBL3874879

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Antagonist activity at human recombinant full length TRPM8 expressed in HEK293 cells assessed as inhibition of icilin-induced calcium increase preincubated for 10 mins followed by icilin addition by Fluo-4 AM dye based FLIPR assay
1
Total Activities
1
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Autocuration

Publication

Synthesis and optimization of novel α-phenylglycinamides as selective TRPM8 antagonists.
Kobayashi JI, Hirasawa H, Ozawa T, Ozawa T, Takeda H, Fujimori Y, Nakanishi O, Kamada N, Ikeda T.
Bioorg Med Chem (2017) 25 : 727 -742
PubMed 27964995 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 1 6.23 6.23

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3900018 1 6.23

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3900018 IC50 = 588.84 nM 6.23