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Assay Detail

CHEMBL3877341

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of full length human recombinant N-terminal GST-tagged HDAC6 (1 to 1215 residues) expressed in sf9 cells preincubated with enzyme followed by fluorogenic Arg-His-Lys-Lys(Ac)-AMC substrate addition measured after 2 hrs by fluorescence assay
21
Total Activities
21
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type Sf9
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Histone deacetylase 6 (CHEMBL1865)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Design and Synthesis of Janus Kinase 2 (JAK2) and Histone Deacetlyase (HDAC) Bispecific Inhibitors Based on Pacritinib and Evidence of Dual Pathway Inhibition in Hematological Cell Lines.
Yang EG, Mustafa N, Tan EC, Poulsen A, Ramanujulu PM, Chng WJ, Yen JJ, Dymock BW.
J Med Chem (2016) 59 : 8233 -8262
PubMed 27541357 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 21 6.57 8.68

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3917405 1 8.68
CHEMBL3972157 1 8.64
CHEMBL3933593 1 8.60
CHEMBL2018302 TUBASTATIN A 1 8.46
CHEMBL3945293 1 7.80
CHEMBL98 VORINOSTAT 4.0 1 7.70
CHEMBL3951291 1 7.55
CHEMBL3924682 1 7.18
CHEMBL3980203 1 6.97
CHEMBL3915689 1 6.40
CHEMBL3942555 1 6.29
CHEMBL3961053 1 6.05
CHEMBL3964125 1 5.58
CHEMBL3920872 1 5.55
CHEMBL3952364 1 5.54
CHEMBL3943650 1 5.00
CHEMBL3892860 1 4.35
CHEMBL3982857 1 4.28
CHEMBL3954183 1 4.19
CHEMBL3893970 1 -
CHEMBL3962918 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3917405 IC50 = 2.1 nM 8.68
CHEMBL3972157 IC50 = 2.3 nM 8.64
CHEMBL3933593 IC50 = 2.5 nM 8.60
CHEMBL2018302 TUBASTATIN A IC50 = 3.5 nM 8.46
CHEMBL3945293 IC50 = 15.8 nM 7.80
CHEMBL98 VORINOSTAT IC50 = 20.0 nM 7.70
CHEMBL3951291 IC50 = 28.0 nM 7.55
CHEMBL3924682 IC50 = 66.0 nM 7.18
CHEMBL3980203 IC50 = 107.0 nM 6.97
CHEMBL3915689 IC50 = 395.0 nM 6.40
CHEMBL3942555 IC50 = 510.0 nM 6.29
CHEMBL3961053 IC50 = 899.0 nM 6.05
CHEMBL3964125 IC50 = 2600.0 nM 5.58
CHEMBL3920872 IC50 = 2800.0 nM 5.55
CHEMBL3952364 IC50 = 2900.0 nM 5.54
CHEMBL3943650 IC50 = 10000.0 nM 5.00
CHEMBL3892860 IC50 = 45000.0 nM 4.35
CHEMBL3982857 IC50 = 53000.0 nM 4.28
CHEMBL3954183 IC50 = 64000.0 nM 4.19
CHEMBL3893970 IC50 > 100000.0 nM -
CHEMBL3962918 IC50 > 100000.0 nM -