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Assay Detail

CHEMBL3877344

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of JAK2 (unknown origin) preincubated for 20 mins followed by [33P]ATP addition measured after 120 mins by Hotspot assay
16
Total Activities
16
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Tyrosine-protein kinase JAK2 (CHEMBL2971)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Design and Synthesis of Janus Kinase 2 (JAK2) and Histone Deacetlyase (HDAC) Bispecific Inhibitors Based on Pacritinib and Evidence of Dual Pathway Inhibition in Hematological Cell Lines.
Yang EG, Mustafa N, Tan EC, Poulsen A, Ramanujulu PM, Chng WJ, Yen JJ, Dymock BW.
J Med Chem (2016) 59 : 8233 -8262
PubMed 27541357 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 16 8.43 9.01

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3961053 1 9.01
CHEMBL2035187 PACRITINIB 4.0 1 9.00
CHEMBL3924682 1 8.90
CHEMBL3917405 1 8.85
CHEMBL3915689 1 8.77
CHEMBL3964125 1 8.64
CHEMBL3980203 1 8.61
CHEMBL3920872 1 8.59
CHEMBL3951291 1 8.37
CHEMBL3942555 1 8.33
CHEMBL3945293 1 8.30
CHEMBL3933593 1 8.16
CHEMBL3952364 1 8.05
CHEMBL3972157 1 7.52
CHEMBL3981879 1 7.33
CHEMBL3954183 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3961053 IC50 = 0.97 nM 9.01
CHEMBL2035187 PACRITINIB IC50 = 1.0 nM 9.00
CHEMBL3924682 IC50 = 1.27 nM 8.90
CHEMBL3917405 IC50 = 1.4 nM 8.85
CHEMBL3915689 IC50 = 1.71 nM 8.77
CHEMBL3964125 IC50 = 2.31 nM 8.64
CHEMBL3980203 IC50 = 2.45 nM 8.61
CHEMBL3920872 IC50 = 2.57 nM 8.59
CHEMBL3951291 IC50 = 4.3 nM 8.37
CHEMBL3942555 IC50 = 4.7 nM 8.33
CHEMBL3945293 IC50 = 5.0 nM 8.30
CHEMBL3933593 IC50 = 6.9 nM 8.16
CHEMBL3952364 IC50 = 9.0 nM 8.05
CHEMBL3972157 IC50 = 30.0 nM 7.52
CHEMBL3981879 IC50 = 47.0 nM 7.33
CHEMBL3954183 IC50 < 39.0 nM -