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Assay Detail

CHEMBL3877552

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Agonist activity at human alpha4beta2 nAChR expressed in human SH-EP1 cells after 9.5 mins by 86Rb+ efflux assay
13
Total Activities
7
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type SH-EP1
Confidence 7 — Homologous single protein target
Curated By Autocuration

Target

Neuronal acetylcholine receptor; alpha4/beta2 (CHEMBL1907589)
Type PROTEIN COMPLEX
Organism Homo sapiens

Publication

Synthesis and biological evaluation of novel hybrids of highly potent and selective α4β2-Nicotinic acetylcholine receptor (nAChR) partial agonists.
Zhang HK, Eaton JB, Fedolak A, Gunosewoyo H, Onajole OK, Brunner D, Lukas RJ, Yu LF, Kozikowski AP.
Eur J Med Chem (2016) 124 : 689 -697
PubMed 27639361 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 13 7.58 7.80

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3948977 2 7.80
CHEMBL3898434 2 7.77
CHEMBL3970672 2 7.51
CHEMBL3926480 1 6.90
CHEMBL3889475 2 -
CHEMBL3899499 2 -
CHEMBL3908556 2 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3948977 EC50 = 15.9 nM 7.80
CHEMBL3948977 EC50 = 15.85 nM 7.80
CHEMBL3898434 EC50 = 17.1 nM 7.77
CHEMBL3898434 EC50 = 16.98 nM 7.77
CHEMBL3970672 EC50 = 30.9 nM 7.51
CHEMBL3970672 EC50 = 30.9 nM 7.51
CHEMBL3926480 EC50 = 125.0 nM 6.90
CHEMBL3889475 EC50 > 1000.0 nM -
CHEMBL3899499 EC50 > 1000.0 nM -
CHEMBL3908556 EC50 > 1000.0 nM -
CHEMBL3889475 EC50 > 1000.0 nM -
CHEMBL3899499 EC50 > 1000.0 nM -
CHEMBL3908556 EC50 > 1000.0 nM -