Skip to main content
Free research Free research Free assay review with research home and workspace preview
Quick search ChEMBL 36
Assay Detail

CHEMBL3877553

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Antagonist activity at human alpha4beta2 expressed in human SH-EP1 cells assessed as reduction in carbamylcholine induced 86Rb+ efflux preincubated for 10 mins followed by carbamylcholine addition measured after 5 mins by Cherenkov counting
20
Total Activities
7
Compounds Tested
2
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type SH-EP1
Confidence 7 — Homologous single protein target
Curated By Autocuration

Target

Neuronal acetylcholine receptor; alpha4/beta2 (CHEMBL1907589)
Type PROTEIN COMPLEX
Organism Homo sapiens

Publication

Synthesis and biological evaluation of novel hybrids of highly potent and selective α4β2-Nicotinic acetylcholine receptor (nAChR) partial agonists.
Zhang HK, Eaton JB, Fedolak A, Gunosewoyo H, Onajole OK, Brunner D, Lukas RJ, Yu LF, Kozikowski AP.
Eur J Med Chem (2016) 124 : 689 -697
PubMed 27639361 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 13 7.51 7.80
Efficacy 7 - -

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3948977 3 7.80
CHEMBL3898434 3 7.80
CHEMBL3970672 3 7.29
CHEMBL3926480 2 6.77
CHEMBL3889475 3 -
CHEMBL3899499 3 -
CHEMBL3908556 3 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3948977 IC50 = 15.85 nM 7.80
CHEMBL3898434 IC50 = 15.85 nM 7.80
CHEMBL3948977 IC50 = 16.0 nM 7.80
CHEMBL3898434 IC50 = 15.7 nM 7.80
CHEMBL3970672 IC50 = 50.8 nM 7.29
CHEMBL3970672 IC50 = 51.29 nM 7.29
CHEMBL3926480 IC50 = 169.0 nM 6.77
CHEMBL3889475 IC50 > 1000.0 nM -
CHEMBL3899499 IC50 > 1000.0 nM -
CHEMBL3908556 IC50 > 1000.0 nM -
CHEMBL3889475 IC50 > 1000.0 nM -
CHEMBL3908556 IC50 > 1000.0 nM -
CHEMBL3948977 Efficacy = 74.0 % -
CHEMBL3889475 Efficacy - -
CHEMBL3926480 Efficacy - -
CHEMBL3898434 Efficacy = 79.0 % -
CHEMBL3899499 Efficacy - -
CHEMBL3970672 Efficacy = 68.0 % -
CHEMBL3908556 Efficacy - -
CHEMBL3899499 IC50 > 1000.0 nM -