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Assay Detail

CHEMBL3880187

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Agonist activity at human S1P2 expressed in CRE-bla CHOK1 cells assessed as inhibition of cAMP formation preincubated for 4 hrs followed by FRET-based beta-lactamase fluorescent substrate addition measured after 2 hrs
14
Total Activities
14
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type CHO-K1
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Sphingosine 1-phosphate receptor 2 (CHEMBL2955)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Selective sphingosine 1 phosphate receptor modulators and methods of chiral synthesis
(2013)

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 14 5.37 5.97

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3902160 1 5.97
CHEMBL3894716 1 5.80
CHEMBL3919445 1 5.26
CHEMBL3951270 1 5.25
CHEMBL3921214 1 5.18
CHEMBL3899384 1 5.11
CHEMBL3707247 OZANIMOD 4.0 1 5.02
CHEMBL3922998 1 -
CHEMBL3933952 1 -
CHEMBL3931243 1 -
CHEMBL3895230 1 -
CHEMBL3910269 1 -
CHEMBL3893172 1 -
CHEMBL3922179 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3902160 EC50 = 1080.0 nM 5.97
CHEMBL3894716 EC50 = 1569.0 nM 5.80
CHEMBL3919445 EC50 = 5500.0 nM 5.26
CHEMBL3951270 EC50 = 5690.0 nM 5.25
CHEMBL3921214 EC50 = 6662.0 nM 5.18
CHEMBL3899384 EC50 = 7717.0 nM 5.11
CHEMBL3707247 OZANIMOD EC50 = 9559.0 nM 5.02
CHEMBL3922998 EC50 > 10000.0 nM -
CHEMBL3933952 EC50 > 10000.0 nM -
CHEMBL3931243 EC50 > 10000.0 nM -
CHEMBL3895230 EC50 > 10000.0 nM -
CHEMBL3910269 EC50 > 10000.0 nM -
CHEMBL3893172 EC50 > 10000.0 nM -
CHEMBL3922179 EC50 > 1000.0 nM -