Assay Detail
Binding
CHEMBL3880966
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of full length human AKT3 expressed in Sf9 cells assessed as reduction in substrate phosphorylation using biotin-ahx-ARKRERAYSFGHHA-amide substrate and [gamma-33P]ATP incubated for 40 mins by top count microplate scintillation counting method
20
Total Activities
20
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Cell Type | Sf9 |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Target
RAC-gamma serine/threonine-protein kinase (CHEMBL4816) ↗| Type | SINGLE PROTEIN |
| Organism | Homo sapiens |
Publication
Inhibitors of AKT Activity
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 20 | 8.60 | 8.80 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL2219422 | AFURESERTIB | 3.0 | 1 | 8.80 |
| CHEMBL3953878 | — | — | 1 | 8.40 |
| CHEMBL3890070 | — | — | 1 | - |
| CHEMBL3952025 | — | — | 1 | - |
| CHEMBL3942525 | — | — | 1 | - |
| CHEMBL3907254 | — | — | 1 | - |
| CHEMBL3904492 | — | — | 1 | - |
| CHEMBL3968297 | — | — | 1 | - |
| CHEMBL3965875 | — | — | 1 | - |
| CHEMBL3926382 | — | — | 1 | - |
| CHEMBL3923631 | — | — | 1 | - |
| CHEMBL3892820 | — | — | 1 | - |
| CHEMBL3963487 | — | — | 1 | - |
| CHEMBL3953883 | — | — | 1 | - |
| CHEMBL3929383 | — | — | 1 | - |
| CHEMBL3932335 | — | — | 1 | - |
| CHEMBL3935057 | — | — | 1 | - |
| CHEMBL3971992 | — | — | 1 | - |
| CHEMBL3903718 | — | — | 1 | - |
| CHEMBL3905729 | — | — | 1 | - |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL2219422 | AFURESERTIB | IC50 | = | 1.585 | nM | 8.80 |
| CHEMBL3953878 | — | IC50 | = | 3.981 | nM | 8.40 |
| CHEMBL3890070 | — | IC50 | <= | 25.12 | nM | - |
| CHEMBL3952025 | — | IC50 | <= | 25.12 | nM | - |
| CHEMBL3942525 | — | IC50 | <= | 25.12 | nM | - |
| CHEMBL3907254 | — | IC50 | <= | 25.12 | nM | - |
| CHEMBL3904492 | — | IC50 | <= | 25.12 | nM | - |
| CHEMBL3968297 | — | IC50 | <= | 25.12 | nM | - |
| CHEMBL3965875 | — | IC50 | <= | 25.12 | nM | - |
| CHEMBL3926382 | — | IC50 | <= | 25.12 | nM | - |
| CHEMBL3923631 | — | IC50 | <= | 25.12 | nM | - |
| CHEMBL3892820 | — | IC50 | <= | 25.12 | nM | - |
| CHEMBL3963487 | — | IC50 | <= | 25.12 | nM | - |
| CHEMBL3953883 | — | IC50 | <= | 25.12 | nM | - |
| CHEMBL3929383 | — | IC50 | <= | 25.12 | nM | - |
| CHEMBL3932335 | — | IC50 | <= | 25.12 | nM | - |
| CHEMBL3935057 | — | IC50 | <= | 25.12 | nM | - |
| CHEMBL3971992 | — | IC50 | <= | 25.12 | nM | - |
| CHEMBL3903718 | — | IC50 | <= | 25.12 | nM | - |
| CHEMBL3905729 | — | IC50 | <= | 25.12 | nM | - |