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Assay Detail

CHEMBL3882094

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of N-terminal GST-tagged recombinant human C-Raf Y340D/Y341D double mutant expressed in baculovirus infected Sf21 insect cells using MEK1 as substrate preincubated for 5 mins followed by substrate addition measured after 30 mins by DELFIA
2
Total Activities
2
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 5 — Multiple protein complex / RNA
Curated By Autocuration

Target

RAF serine/threonine protein kinase (CHEMBL3559685)
Type PROTEIN FAMILY
Organism Homo sapiens

Publication

Multi-functional small molecules as anti-proliferative agents
(2008)

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 2 6.00 6.00

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3934468 1 6.00
CHEMBL3946124 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3934468 IC50 = 1000.0 nM 6.00
CHEMBL3946124 IC50 <= 100.0 nM -