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Assay Detail

CHEMBL3882633

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of African green monkey ACAT2 expressed in mouse AC29 cells assessed as reduction in esterification of NBD-cholesterol preincubated for 20 mins followed by NBD-cholesterol addition measured after 6 hrs by high-performance TLC method
4
Total Activities
4
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Chlorocebus aethiops
Cell Type AC29
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Sterol O-acyltransferase 2 (CHEMBL3879854)
Type SINGLE PROTEIN
Organism Chlorocebus aethiops

Publication

Method for selectively inhibiting ACAT1 in the treatment of neurodegenerative diseases
(2014)

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 4 6.22 6.56

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3894947 1 6.56
CHEMBL3899908 1 6.28
CHEMBL3936899 1 6.22
CHEMBL3905226 1 5.82

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3894947 IC50 = 275.0 nM 6.56
CHEMBL3899908 IC50 = 530.0 nM 6.28
CHEMBL3936899 IC50 = 600.0 nM 6.22
CHEMBL3905226 IC50 = 1500.0 nM 5.82