Assay Detail
Binding
CHEMBL3887103
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Enzyme Assay: A convenient method to determine IC50 of a PARP inhibitor compound is a PARP assay using purified recombinant human PARP from Trevigan (Gaithersburg, Md.), as follows: The PARP enzyme assay is set up on ice in a volume of 100 microliters consisting of 100 mM Tris-HCl (pH 8.0), 1 mM MgCl2, 28 mM KCl, 28 mM NaCl, 3.0 μg/ml of DNase I-activated herring sperm DNA (Sigma, Mo.), 30 micromolar [3H]nicotinamide adenine dinucleotide (62.5 mci/mmole), 15 micrograms/ml PARP enzyme, and various concentrations of the compounds to be tested. The reaction is initiated by adding enzyme and incubating the mixture at 25° C. After 2 minutes of incubation, the reaction is terminated by adding 500 microliters of ice cold 30% (w/v) trichloroacetic acid. The precipitate formed is transferred onto a glass fiber filter (Packard Unifilter-GF/C) and washed three times with 70% ethanol. After the filter is dried, the radioactivity is determined by scintillation counting.
59
Total Activities
57
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Confidence | 8 — Homologous single protein target |
| Curated By | Autocuration |
Publication
PARP inhibitor compounds, compositions and methods of use
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 59 | 7.62 | 8.22 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL3644564 | — | — | 1 | 8.22 |
| CHEMBL3644565 | — | — | 1 | 8.05 |
| CHEMBL3644616 | — | — | 1 | 8.00 |
| CHEMBL3644596 | — | — | 1 | 7.96 |
| CHEMBL3644587 | 2X-121 | 2.0 | 1 | 7.92 |
| CHEMBL3644566 | — | — | 1 | 7.92 |
| CHEMBL3644605 | — | — | 1 | 7.89 |
| CHEMBL3644592 | — | — | 1 | 7.89 |
| CHEMBL3644581 | — | — | 1 | 7.89 |
| CHEMBL3644601 | — | — | 1 | 7.85 |
| CHEMBL3644598 | — | — | 1 | 7.82 |
| CHEMBL3644589 | — | — | 1 | 7.82 |
| CHEMBL3644610 | — | — | 1 | 7.82 |
| CHEMBL3644604 | — | — | 1 | 7.77 |
| CHEMBL3644571 | — | — | 1 | 7.77 |
| CHEMBL3644572 | — | — | 1 | 7.75 |
| CHEMBL3644567 | — | — | 1 | 7.75 |
| CHEMBL3644602 | — | — | 1 | 7.75 |
| CHEMBL3644606 | — | — | 2 | 7.72 |
| CHEMBL3644563 | — | — | 1 | 7.72 |
| CHEMBL3644586 | — | — | 1 | 7.72 |
| CHEMBL3644588 | — | — | 1 | 7.70 |
| CHEMBL3644570 | — | — | 1 | 7.70 |
| CHEMBL3644569 | — | — | 1 | 7.68 |
| CHEMBL3644599 | — | — | 1 | 7.68 |
| CHEMBL3644594 | — | — | 1 | 7.68 |
| CHEMBL3644611 | — | — | 1 | 7.66 |
| CHEMBL3644609 | — | — | 1 | 7.66 |
| CHEMBL3644561 | — | — | 1 | 7.64 |
| CHEMBL3644615 | — | — | 2 | 7.64 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL3644564 | — | IC50 | = | 6.0 | nM | 8.22 |
| CHEMBL3644565 | — | IC50 | = | 9.0 | nM | 8.05 |
| CHEMBL3644616 | — | IC50 | = | 10.0 | nM | 8.00 |
| CHEMBL3644596 | — | IC50 | = | 11.0 | nM | 7.96 |
| CHEMBL3644566 | — | IC50 | = | 12.0 | nM | 7.92 |
| CHEMBL3644587 | 2X-121 | IC50 | = | 12.0 | nM | 7.92 |
| CHEMBL3644605 | — | IC50 | = | 13.0 | nM | 7.89 |
| CHEMBL3644592 | — | IC50 | = | 13.0 | nM | 7.89 |
| CHEMBL3644581 | — | IC50 | = | 13.0 | nM | 7.89 |
| CHEMBL3644601 | — | IC50 | = | 14.0 | nM | 7.85 |
| CHEMBL3644589 | — | IC50 | = | 15.0 | nM | 7.82 |
| CHEMBL3644610 | — | IC50 | = | 15.0 | nM | 7.82 |
| CHEMBL3644598 | — | IC50 | = | 15.0 | nM | 7.82 |
| CHEMBL3644571 | — | IC50 | = | 17.0 | nM | 7.77 |
| CHEMBL3644604 | — | IC50 | = | 17.0 | nM | 7.77 |
| CHEMBL3644567 | — | IC50 | = | 18.0 | nM | 7.75 |
| CHEMBL3644572 | — | IC50 | = | 18.0 | nM | 7.75 |
| CHEMBL3644602 | — | IC50 | = | 18.0 | nM | 7.75 |
| CHEMBL3644606 | — | IC50 | = | 19.0 | nM | 7.72 |
| CHEMBL3644586 | — | IC50 | = | 19.0 | nM | 7.72 |
| CHEMBL3644563 | — | IC50 | = | 19.0 | nM | 7.72 |
| CHEMBL3644570 | — | IC50 | = | 20.0 | nM | 7.70 |
| CHEMBL3644588 | — | IC50 | = | 20.0 | nM | 7.70 |
| CHEMBL3644594 | — | IC50 | = | 21.0 | nM | 7.68 |
| CHEMBL3644599 | — | IC50 | = | 21.0 | nM | 7.68 |
| CHEMBL3644569 | — | IC50 | = | 21.0 | nM | 7.68 |
| CHEMBL3644609 | — | IC50 | = | 22.0 | nM | 7.66 |
| CHEMBL3644611 | — | IC50 | = | 22.0 | nM | 7.66 |
| CHEMBL3644606 | — | IC50 | = | 23.0 | nM | 7.64 |
| CHEMBL3644600 | — | IC50 | = | 23.0 | nM | 7.64 |
| CHEMBL3644615 | — | IC50 | = | 23.0 | nM | 7.64 |
| CHEMBL3644561 | — | IC50 | = | 23.0 | nM | 7.64 |
| CHEMBL3644607 | — | IC50 | = | 24.0 | nM | 7.62 |
| CHEMBL3644577 | — | IC50 | = | 26.0 | nM | 7.58 |
| CHEMBL3644608 | — | IC50 | = | 27.0 | nM | 7.57 |
| CHEMBL3644603 | — | IC50 | = | 27.0 | nM | 7.57 |
| CHEMBL3644582 | — | IC50 | = | 28.0 | nM | 7.55 |
| CHEMBL3644580 | — | IC50 | = | 29.0 | nM | 7.54 |
| CHEMBL3644615 | — | IC50 | = | 30.0 | nM | 7.52 |
| CHEMBL3644583 | — | IC50 | = | 31.0 | nM | 7.51 |
| CHEMBL3644568 | — | IC50 | = | 32.0 | nM | 7.50 |
| CHEMBL3644573 | — | IC50 | = | 35.0 | nM | 7.46 |
| CHEMBL3644574 | — | IC50 | = | 35.0 | nM | 7.46 |
| CHEMBL3639434 | — | IC50 | = | 35.0 | nM | 7.46 |
| CHEMBL3644562 | — | IC50 | = | 35.0 | nM | 7.46 |
| CHEMBL3644593 | — | IC50 | = | 38.0 | nM | 7.42 |
| CHEMBL3644575 | — | IC50 | = | 39.0 | nM | 7.41 |
| CHEMBL3644590 | — | IC50 | = | 39.0 | nM | 7.41 |
| CHEMBL3644578 | — | IC50 | = | 41.0 | nM | 7.39 |
| CHEMBL3644591 | — | IC50 | = | 42.0 | nM | 7.38 |