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Assay Detail

CHEMBL3887128

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Time Resolved-Fluorescence Resonance Energy Transfer (TR-FRET) Assay: The inhibition constant (Ki) is the dissociation constant of an enzyme-inhibitor complex or a protein/small molecule complex, wherein the small molecule is inhibiting binding of one protein to another protein or peptide. Where the Ki for a compound is represented as > (greater than) a certain numerical value, it is intended to mean that the binding affinity value (e.g., for Bcl-XL) is greater than the limits of detection of the assay used. Where the binding selectivity ratio for a compound is represented as > (greater than) a certain numerical value, it is intended to mean that the selectivity of a particular compound for Bcl-2 over Bcl-XL is at least as great as the number indicated. Where the Ki for a compound is represented as < (less than) a certain numerical value, it is intended to mean that the binding affinity value (e.g., for Bcl-2) is lower than the limit of detection of the assay used. Inhibition constants were determined using Wang's equation (Wang Z-X).
13
Total Activities
13
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 8 — Homologous single protein target
Curated By Autocuration

Target

Apoptosis regulator Bcl-2 (CHEMBL4860)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Bcl-2-selective apoptosis-inducing agents for the treatment of cancer and immune diseases
(2015)

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 13 10.18 10.85

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4110818 1 10.85
CHEMBL4111550 1 10.44
CHEMBL3903167 1 10.44
CHEMBL3137309 VENETOCLAX 4.0 1 10.44
CHEMBL3961062 1 10.15
CHEMBL3930583 1 8.78
CHEMBL3986326 1 -
CHEMBL3978114 1 -
CHEMBL4114397 1 -
CHEMBL4109545 1 -
CHEMBL3915032 1 -
CHEMBL3900610 1 -
CHEMBL3925160 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4110818 Ki = 0.014 nM 10.85
CHEMBL4111550 Ki = 0.036 nM 10.44
CHEMBL3903167 Ki = 0.036 nM 10.44
CHEMBL3137309 VENETOCLAX Ki = 0.036 nM 10.44
CHEMBL3961062 Ki = 0.071 nM 10.15
CHEMBL3930583 Ki = 1.654 nM 8.78
CHEMBL3986326 Ki < 0.01 nM -
CHEMBL3978114 Ki < 0.01 nM -
CHEMBL4114397 Ki < 0.01 nM -
CHEMBL4109545 Ki < 0.01 nM -
CHEMBL3915032 Ki < 0.01 nM -
CHEMBL3900610 Ki < 0.01 nM -
CHEMBL3925160 Ki < 0.01 nM -