Assay Detail
Binding
CHEMBL3887166
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Reporter Assay: For TLR8 and TLR7 activity testing, HEK-Blue human TLR8 or TLR7 cells, respectively, (Invivogen, San Diego, Calif., USA) transfected with a SEAP reporter (secreted embryonic alkaline phosphatase) construct were used, in which the reporter expression is regulated by the NF-κB promoter upon stimulation for 24 hr. The reporter activity was determined using Quanti Blue kit (Invivogen, San Diego, Calif., USA) at a wavelength of 640 nm.
13
Total Activities
13
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Confidence | 8 — Homologous single protein target |
| Curated By | Autocuration |
Publication
4-amino-imidazoquinoline compounds
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| EC50 | 13 | 6.86 | 7.57 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL3924480 | — | — | 1 | 7.57 |
| CHEMBL3921751 | — | — | 1 | 7.30 |
| CHEMBL3967031 | — | — | 1 | 7.26 |
| CHEMBL3904306 | — | — | 1 | 7.22 |
| CHEMBL3960305 | — | — | 1 | 6.89 |
| CHEMBL3984333 | — | — | 1 | 6.87 |
| CHEMBL3975333 | — | — | 1 | 6.86 |
| CHEMBL3959973 | — | — | 1 | 6.82 |
| CHEMBL3944005 | — | — | 1 | 6.71 |
| CHEMBL3942013 | — | — | 1 | 6.65 |
| CHEMBL383322 | RESIQUIMOD | 2.0 | 1 | 6.12 |
| CHEMBL3964358 | — | — | 1 | 6.05 |
| CHEMBL3915625 | — | — | 1 | - |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL3924480 | — | EC50 | = | 27.0 | nM | 7.57 |
| CHEMBL3921751 | — | EC50 | = | 50.0 | nM | 7.30 |
| CHEMBL3967031 | — | EC50 | = | 55.0 | nM | 7.26 |
| CHEMBL3904306 | — | EC50 | = | 60.0 | nM | 7.22 |
| CHEMBL3960305 | — | EC50 | = | 130.0 | nM | 6.89 |
| CHEMBL3984333 | — | EC50 | = | 134.0 | nM | 6.87 |
| CHEMBL3975333 | — | EC50 | = | 139.0 | nM | 6.86 |
| CHEMBL3959973 | — | EC50 | = | 152.0 | nM | 6.82 |
| CHEMBL3944005 | — | EC50 | = | 195.0 | nM | 6.71 |
| CHEMBL3942013 | — | EC50 | = | 223.0 | nM | 6.65 |
| CHEMBL383322 | RESIQUIMOD | EC50 | = | 760.0 | nM | 6.12 |
| CHEMBL3964358 | — | EC50 | = | 900.0 | nM | 6.05 |
| CHEMBL3915625 | — | EC50 | > | 100000.0 | nM | - |