Assay Detail
Binding
CHEMBL3887262
Review assay metadata, readout intent, target linkage, and publication context from the same page.
CDK9/CycT1 Kinase Assay: Recombinant full-length His-tagged human CDK9 and CycT1, expressed in insect cells and purified by Ni-NTA affinity chromatography, were purchased from Invitrogen (Cat. No PV4131). As substrate for the kinase reaction biotinylated peptide biotin-Ttds-YISPLKSPYKISEG (C-terminus in amid form) was used which can be purchased e.g. form the company JERINI Peptide Technologies (Berlin, Germany) For the assay 50 nl of a 100 fold concentrated solution of the test compound in DMSO was pipetted into a black low volume 384 well microtiter plate (Greiner Bio-One, Frickenhausen, Germany), 2 ul of a solution of CDK9/CycT1 in aqueous assay buffer [50 mM Tris/HCl pH 8.0, 10 mM MgCl2, 1.0 mM dithiothreitol, 0.1 mM sodium ortho-vanadate, 0.01% (v/v) Nonidet-P40 (Sigma)] were added and the mixture was incubated for 15 min at 22 C. to allow pre-binding of the test compounds to the enzyme before the start of the kinase reaction.
63
Total Activities
39
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Confidence | 8 — Homologous single protein target |
| Curated By | Autocuration |
Publication
Disubstituted 5-fluoro pyrimidine derivatives containing a sulfoximine group
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 63 | 8.26 | 8.70 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL3973367 | — | — | 1 | 8.70 |
| CHEMBL3905335 | — | — | 1 | 8.70 |
| CHEMBL3959283 | — | — | 1 | 8.70 |
| CHEMBL3944304 | — | — | 1 | 8.70 |
| CHEMBL3979266 | — | — | 1 | 8.70 |
| CHEMBL3946675 | — | — | 3 | 8.70 |
| CHEMBL3932396 | — | — | 1 | 8.52 |
| CHEMBL3960677 | — | — | 3 | 8.52 |
| CHEMBL3960348 | — | — | 3 | 8.52 |
| CHEMBL3953160 | — | — | 1 | 8.52 |
| CHEMBL4115909 | — | — | 3 | 8.52 |
| CHEMBL3934141 | — | — | 1 | 8.40 |
| CHEMBL3959867 | — | — | 3 | 8.40 |
| CHEMBL3937866 | — | — | 1 | 8.40 |
| CHEMBL3919796 | — | — | 1 | 8.40 |
| CHEMBL3897217 | — | — | 3 | 8.40 |
| CHEMBL3967588 | — | — | 1 | 8.40 |
| CHEMBL3917892 | — | — | 3 | 8.40 |
| CHEMBL3892881 | — | — | 1 | 8.30 |
| CHEMBL3981911 | — | — | 1 | 8.30 |
| CHEMBL3923419 | — | — | 1 | 8.30 |
| CHEMBL3976450 | — | — | 3 | 8.30 |
| CHEMBL3965161 | — | — | 3 | 8.30 |
| CHEMBL3962241 | — | — | 3 | 8.30 |
| CHEMBL3912866 | — | — | 1 | 8.22 |
| CHEMBL3949630 | — | — | 1 | 8.22 |
| CHEMBL4115908 | — | — | 1 | 8.22 |
| CHEMBL3916775 | — | — | 1 | 8.15 |
| CHEMBL3934257 | — | — | 3 | 8.15 |
| CHEMBL3934755 | — | — | 1 | 8.10 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL3959283 | — | IC50 | = | 2.0 | nM | 8.70 |
| CHEMBL3946675 | — | IC50 | = | 2.0 | nM | 8.70 |
| CHEMBL3944304 | — | IC50 | = | 2.0 | nM | 8.70 |
| CHEMBL3979266 | — | IC50 | = | 2.0 | nM | 8.70 |
| CHEMBL3905335 | — | IC50 | = | 2.0 | nM | 8.70 |
| CHEMBL3973367 | — | IC50 | = | 2.0 | nM | 8.70 |
| CHEMBL3960677 | — | IC50 | = | 3.0 | nM | 8.52 |
| CHEMBL4115909 | — | IC50 | = | 3.0 | nM | 8.52 |
| CHEMBL3953160 | — | IC50 | = | 3.0 | nM | 8.52 |
| CHEMBL3946675 | — | IC50 | = | 3.0 | nM | 8.52 |
| CHEMBL3960348 | — | IC50 | = | 3.0 | nM | 8.52 |
| CHEMBL3932396 | — | IC50 | = | 3.0 | nM | 8.52 |
| CHEMBL3960677 | — | IC50 | = | 4.0 | nM | 8.40 |
| CHEMBL3934141 | — | IC50 | = | 4.0 | nM | 8.40 |
| CHEMBL3897217 | — | IC50 | = | 4.0 | nM | 8.40 |
| CHEMBL3897217 | — | IC50 | = | 4.0 | nM | 8.40 |
| CHEMBL3919796 | — | IC50 | = | 4.0 | nM | 8.40 |
| CHEMBL3967588 | — | IC50 | = | 4.0 | nM | 8.40 |
| CHEMBL3917892 | — | IC50 | = | 4.0 | nM | 8.40 |
| CHEMBL3946675 | — | IC50 | = | 4.0 | nM | 8.40 |
| CHEMBL3937866 | — | IC50 | = | 4.0 | nM | 8.40 |
| CHEMBL3960677 | — | IC50 | = | 4.0 | nM | 8.40 |
| CHEMBL3959867 | — | IC50 | = | 4.0 | nM | 8.40 |
| CHEMBL3981911 | — | IC50 | = | 5.0 | nM | 8.30 |
| CHEMBL3965161 | — | IC50 | = | 5.0 | nM | 8.30 |
| CHEMBL3923419 | — | IC50 | = | 5.0 | nM | 8.30 |
| CHEMBL3959867 | — | IC50 | = | 5.0 | nM | 8.30 |
| CHEMBL3962241 | — | IC50 | = | 5.0 | nM | 8.30 |
| CHEMBL3892881 | — | IC50 | = | 5.0 | nM | 8.30 |
| CHEMBL3976450 | — | IC50 | = | 5.0 | nM | 8.30 |
| CHEMBL4115909 | — | IC50 | = | 5.0 | nM | 8.30 |
| CHEMBL3917892 | — | IC50 | = | 5.0 | nM | 8.30 |
| CHEMBL3962241 | — | IC50 | = | 6.0 | nM | 8.22 |
| CHEMBL3897217 | — | IC50 | = | 6.0 | nM | 8.22 |
| CHEMBL3959867 | — | IC50 | = | 6.0 | nM | 8.22 |
| CHEMBL3912866 | — | IC50 | = | 6.0 | nM | 8.22 |
| CHEMBL3949630 | — | IC50 | = | 6.0 | nM | 8.22 |
| CHEMBL4115908 | — | IC50 | = | 6.0 | nM | 8.22 |
| CHEMBL4115909 | — | IC50 | = | 6.0 | nM | 8.22 |
| CHEMBL3917892 | — | IC50 | = | 6.0 | nM | 8.22 |
| CHEMBL3976450 | — | IC50 | = | 7.0 | nM | 8.15 |
| CHEMBL3960348 | — | IC50 | = | 7.0 | nM | 8.15 |
| CHEMBL3934257 | — | IC50 | = | 7.0 | nM | 8.15 |
| CHEMBL3916775 | — | IC50 | = | 7.0 | nM | 8.15 |
| CHEMBL3965161 | — | IC50 | = | 7.0 | nM | 8.15 |
| CHEMBL3976450 | — | IC50 | = | 7.0 | nM | 8.15 |
| CHEMBL3906489 | — | IC50 | = | 8.0 | nM | 8.10 |
| CHEMBL3978185 | — | IC50 | = | 8.0 | nM | 8.10 |
| CHEMBL3960348 | — | IC50 | = | 8.0 | nM | 8.10 |
| CHEMBL3934755 | — | IC50 | = | 8.0 | nM | 8.10 |