Assay Detail
Binding
CHEMBL3887581
Review assay metadata, readout intent, target linkage, and publication context from the same page.
In vitro MMP-8 Assay: The synthetic octapeptide substrate, containing the collagenase-susceptible glycine-isoleucine peptide bond, was incubated (37° C.) with commercially-available chromatrographically-purified human neutrophil collagenase (MMP-8) in the presence of 1 mM Ca++ and the tripeptide degradation fragment and undegraded substrate were separated and measured by HPLC (Waters Alliance 2695 System with a reverse phase C-18 column).
8
Total Activities
8
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Confidence | 8 — Homologous single protein target |
| Curated By | Autocuration |
Publication
Curcumin analogues as zinc chelators and their uses
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 8 | 4.92 | 5.40 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL415879 | PHENANTHROLINE | — | 1 | 5.40 |
| CHEMBL3922961 | — | — | 1 | 5.10 |
| CHEMBL3915135 | — | — | 1 | 4.82 |
| CHEMBL3921145 | — | — | 1 | 4.35 |
| CHEMBL3892642 | — | — | 1 | - |
| CHEMBL3963341 | — | — | 1 | - |
| CHEMBL3962579 | — | — | 1 | - |
| CHEMBL3893144 | — | — | 1 | - |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL415879 | PHENANTHROLINE | IC50 | = | 4000.0 | nM | 5.40 |
| CHEMBL3922961 | — | IC50 | = | 8000.0 | nM | 5.10 |
| CHEMBL3915135 | — | IC50 | = | 15000.0 | nM | 4.82 |
| CHEMBL3921145 | — | IC50 | = | 45000.0 | nM | 4.35 |
| CHEMBL3892642 | — | IC50 | = | 110000.0 | nM | - |
| CHEMBL3963341 | — | IC50 | = | 250000.0 | nM | - |
| CHEMBL3962579 | — | IC50 | < | 1000.0 | nM | - |
| CHEMBL3893144 | — | IC50 | < | 1000.0 | nM | - |