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Assay Detail

CHEMBL3887791

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Radioligand Dose-Displacement Binding Assay: Delta-opioid Receptor Binding Assay Procedures were conducted as follows. Radioligand dose-displacement assays used 0.3 nM [3H]-Naltrindole (Perkin Elmer, Shelton, Conn.; 33.0 Ci/mmole) with 5 ug membrane protein (Perkin Elmer, Shelton, Conn.) in a final volume of 500 ul binding buffer (5 mM MgCl2, 5% DMSO, 50 mM Trizma base, pH 7.4). Non-specific binding was determined in the presence of 25 uM unlabeled naloxone. All reactions were performed in 96-deep well polypropylene plates for 1 hr at a temperature of about 25° C. Binding reactions were terminated by rapid filtration onto 96-well Unifilter GF/C filter plates (Perkin Elmer, Shelton, Conn.) presoaked in 0.5% polyethylenimine (Sigma). Harvesting was performed using a 96-well tissue harvester (Perkin Elmer, Shelton, Conn.) followed by five filtration washes with 500 ul ice-cold binding buffer. Filter plates were subsequently dried at 50° C. for 1-2 hours.
54
Total Activities
54
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 8 — Homologous single protein target
Curated By Autocuration

Target

Delta-type opioid receptor (CHEMBL236)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Buprenorphine analogs
(2015)

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 54 6.37 8.91

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4115222 1 8.91
CHEMBL4110639 1 8.62
CHEMBL4111231 1 7.86
CHEMBL3916122 1 7.82
CHEMBL4111124 1 7.60
CHEMBL4111224 1 7.49
CHEMBL4115091 1 7.37
CHEMBL4111717 1 7.20
CHEMBL4107918 1 7.09
CHEMBL4107906 1 7.03
CHEMBL4107681 1 6.92
CHEMBL4107627 1 6.86
CHEMBL4107821 1 6.82
CHEMBL4109061 1 6.78
CHEMBL4115338 1 6.76
CHEMBL4112472 1 6.74
CHEMBL4114477 1 6.73
CHEMBL4108017 1 6.71
CHEMBL4113804 1 6.68
CHEMBL4110312 1 6.57
CHEMBL4106985 1 6.56
CHEMBL4107470 1 6.50
CHEMBL4112656 1 6.47
CHEMBL4111244 1 6.45
CHEMBL4108066 1 6.42
CHEMBL4112771 1 6.41
CHEMBL4107680 1 6.39
CHEMBL4106544 1 6.33
CHEMBL4109142 1 6.33
CHEMBL4113058 1 6.31

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4115222 Ki = 1.23 nM 8.91
CHEMBL4110639 Ki = 2.38 nM 8.62
CHEMBL4111231 Ki = 13.81 nM 7.86
CHEMBL3916122 Ki = 15.01 nM 7.82
CHEMBL4111124 Ki = 25.38 nM 7.60
CHEMBL4111224 Ki = 32.24 nM 7.49
CHEMBL4115091 Ki = 43.05 nM 7.37
CHEMBL4111717 Ki = 63.02 nM 7.20
CHEMBL4107918 Ki = 81.93 nM 7.09
CHEMBL4107906 Ki = 92.5 nM 7.03
CHEMBL4107681 Ki = 118.99 nM 6.92
CHEMBL4107627 Ki = 138.07 nM 6.86
CHEMBL4107821 Ki = 151.0 nM 6.82
CHEMBL4109061 Ki = 165.88 nM 6.78
CHEMBL4115338 Ki = 174.45 nM 6.76
CHEMBL4112472 Ki = 180.4 nM 6.74
CHEMBL4114477 Ki = 184.83 nM 6.73
CHEMBL4108017 Ki = 194.89 nM 6.71
CHEMBL4113804 Ki = 211.03 nM 6.68
CHEMBL4110312 Ki = 269.62 nM 6.57
CHEMBL4106985 Ki = 277.58 nM 6.56
CHEMBL4107470 Ki = 317.88 nM 6.50
CHEMBL4112656 Ki = 341.52 nM 6.47
CHEMBL4111244 Ki = 355.13 nM 6.45
CHEMBL4108066 Ki = 380.74 nM 6.42
CHEMBL4112771 Ki = 387.09 nM 6.41
CHEMBL4107680 Ki = 410.59 nM 6.39
CHEMBL4106544 Ki = 468.09 nM 6.33
CHEMBL4109142 Ki = 469.9 nM 6.33
CHEMBL4113058 Ki = 493.55 nM 6.31
CHEMBL4109220 Ki = 517.28 nM 6.29
CHEMBL4111378 Ki = 617.83 nM 6.21
CHEMBL4114002 Ki = 667.33 nM 6.18
CHEMBL4112051 Ki = 729.1 nM 6.14
CHEMBL4111803 Ki = 739.45 nM 6.13
CHEMBL4111233 Ki = 743.43 nM 6.13
CHEMBL4115652 Ki = 1004.92 nM 6.00
CHEMBL4109778 Ki = 1054.82 nM 5.98
CHEMBL4112155 Ki = 1479.53 nM 5.83
CHEMBL3930597 Ki = 1704.16 nM 5.77
CHEMBL4108301 Ki = 1732.46 nM 5.76
CHEMBL4109389 Ki = 1983.26 nM 5.70
CHEMBL4112358 Ki = 2425.67 nM 5.62
CHEMBL4113830 Ki = 3024.75 nM 5.52
CHEMBL4114523 Ki = 3001.22 nM 5.52
CHEMBL4113221 Ki = 3254.47 nM 5.49
CHEMBL4107537 Ki = 3615.75 nM 5.44
CHEMBL4107210 Ki = 3710.17 nM 5.43
CHEMBL4110440 Ki = 4096.83 nM 5.39
CHEMBL4106700 Ki = 5530.06 nM 5.26