Assay Detail
Binding
CHEMBL3888198
Review assay metadata, readout intent, target linkage, and publication context from the same page.
HDAC Activity Assay: HDAC assay is performed using fluorescently-labeled acetylated substrate, which comprises an acetylated lysine side chain. After incubation with HDAC, deacetylation of the substrate sensitizes the substrate such that, in a second step, treatment with the detection enzyme produces a fluorophore. HDACs 1 and 6 were expressed as full length fusion proteins. Purified proteins were incubated with 50 μM fluorescently-labeled acetylated peptide substrate and test compound for 2 hours at RT in HDAC assay buffer containing 50 mM Tris-HCl (pH 8.0), 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, 1% DMSO, and 1% BSA. Reactions were terminated by the addition of the Developer after 2 hours, and the development of fluorescence signal, which was relative to the amount of deacetylated peptide, was monitored by time-course measurement of EnVision (PerkinElmer). The HDAC activity was estimated from the slope of time-course measurement of the fluorescence intensity.
51
Total Activities
50
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Confidence | 8 — Homologous single protein target |
| Curated By | Autocuration |
Publication
HDAC inhibitors and therapeutic methods using the same
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 51 | 7.52 | 9.77 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL3912061 | — | — | 1 | 9.77 |
| CHEMBL3960564 | — | — | 1 | 8.84 |
| CHEMBL3960067 | — | — | 1 | 8.84 |
| CHEMBL3951328 | — | — | 1 | 8.60 |
| CHEMBL3893799 | — | — | 1 | 8.60 |
| CHEMBL3944582 | — | — | 1 | 8.53 |
| CHEMBL3927240 | — | — | 1 | 8.53 |
| CHEMBL3909186 | — | — | 1 | 8.46 |
| CHEMBL4113837 | — | — | 1 | 8.46 |
| CHEMBL2018302 | TUBASTATIN A | — | 1 | 8.40 |
| CHEMBL3971436 | — | — | 1 | 8.39 |
| CHEMBL3979548 | — | — | 1 | 8.29 |
| CHEMBL3910971 | — | — | 1 | 8.29 |
| CHEMBL3918660 | — | — | 1 | 8.04 |
| CHEMBL3958794 | — | — | 1 | 8.01 |
| CHEMBL3923615 | — | — | 1 | 8.01 |
| CHEMBL3970950 | — | — | 1 | 7.96 |
| CHEMBL3960054 | — | — | 1 | 7.96 |
| CHEMBL3984046 | — | — | 1 | 7.92 |
| CHEMBL3958552 | — | — | 1 | 7.92 |
| CHEMBL3907295 | — | — | 1 | 7.90 |
| CHEMBL3902014 | — | — | 1 | 7.90 |
| CHEMBL3909854 | — | — | 1 | 7.85 |
| CHEMBL4110772 | — | — | 1 | 7.85 |
| CHEMBL356769 | TUBACIN | — | 1 | 7.82 |
| CHEMBL3927508 | — | — | 1 | 7.53 |
| CHEMBL3948680 | — | — | 2 | 7.53 |
| CHEMBL3973046 | — | — | 1 | 7.53 |
| CHEMBL3934546 | — | — | 1 | 7.50 |
| CHEMBL3976023 | — | — | 1 | 7.50 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL3912061 | — | IC50 | = | 0.17 | nM | 9.77 |
| CHEMBL3960564 | — | IC50 | = | 1.46 | nM | 8.84 |
| CHEMBL3960067 | — | IC50 | = | 1.46 | nM | 8.84 |
| CHEMBL3951328 | — | IC50 | = | 2.51 | nM | 8.60 |
| CHEMBL3893799 | — | IC50 | = | 2.51 | nM | 8.60 |
| CHEMBL3944582 | — | IC50 | = | 2.94 | nM | 8.53 |
| CHEMBL3927240 | — | IC50 | = | 2.94 | nM | 8.53 |
| CHEMBL3909186 | — | IC50 | = | 3.48 | nM | 8.46 |
| CHEMBL4113837 | — | IC50 | = | 3.48 | nM | 8.46 |
| CHEMBL2018302 | TUBASTATIN A | IC50 | = | 4.0 | nM | 8.40 |
| CHEMBL3971436 | — | IC50 | = | 4.08 | nM | 8.39 |
| CHEMBL3979548 | — | IC50 | = | 5.11 | nM | 8.29 |
| CHEMBL3910971 | — | IC50 | = | 5.11 | nM | 8.29 |
| CHEMBL3918660 | — | IC50 | = | 9.2 | nM | 8.04 |
| CHEMBL3923615 | — | IC50 | = | 9.75 | nM | 8.01 |
| CHEMBL3958794 | — | IC50 | = | 9.75 | nM | 8.01 |
| CHEMBL3970950 | — | IC50 | = | 10.9 | nM | 7.96 |
| CHEMBL3960054 | — | IC50 | = | 10.9 | nM | 7.96 |
| CHEMBL3984046 | — | IC50 | = | 11.9 | nM | 7.92 |
| CHEMBL3958552 | — | IC50 | = | 11.9 | nM | 7.92 |
| CHEMBL3907295 | — | IC50 | = | 12.5 | nM | 7.90 |
| CHEMBL3902014 | — | IC50 | = | 12.5 | nM | 7.90 |
| CHEMBL3909854 | — | IC50 | = | 14.2 | nM | 7.85 |
| CHEMBL4110772 | — | IC50 | = | 14.2 | nM | 7.85 |
| CHEMBL356769 | TUBACIN | IC50 | = | 15.0 | nM | 7.82 |
| CHEMBL3927508 | — | IC50 | = | 29.4 | nM | 7.53 |
| CHEMBL3973046 | — | IC50 | = | 29.3 | nM | 7.53 |
| CHEMBL3948680 | — | IC50 | = | 29.3 | nM | 7.53 |
| CHEMBL3976023 | — | IC50 | = | 31.3 | nM | 7.50 |
| CHEMBL3934546 | — | IC50 | = | 31.3 | nM | 7.50 |
| CHEMBL3948680 | — | IC50 | = | 35.0 | nM | 7.46 |
| CHEMBL3898702 | — | IC50 | = | 35.8 | nM | 7.45 |
| CHEMBL3907669 | — | IC50 | = | 35.8 | nM | 7.45 |
| CHEMBL3926575 | — | IC50 | = | 47.0 | nM | 7.33 |
| CHEMBL3936740 | — | IC50 | = | 47.0 | nM | 7.33 |
| CHEMBL3910885 | — | IC50 | = | 70.1 | nM | 7.15 |
| CHEMBL3953441 | — | IC50 | = | 124.0 | nM | 6.91 |
| CHEMBL3899275 | — | IC50 | = | 124.0 | nM | 6.91 |
| CHEMBL3952983 | — | IC50 | = | 126.0 | nM | 6.90 |
| CHEMBL3940062 | — | IC50 | = | 150.0 | nM | 6.82 |
| CHEMBL3938052 | — | IC50 | = | 150.0 | nM | 6.82 |
| CHEMBL3921742 | — | IC50 | = | 540.0 | nM | 6.27 |
| CHEMBL3918458 | — | IC50 | = | 540.0 | nM | 6.27 |
| CHEMBL4107832 | — | IC50 | = | 1250.0 | nM | 5.90 |
| CHEMBL4109326 | — | IC50 | = | 1250.0 | nM | 5.90 |
| CHEMBL3922129 | — | IC50 | = | 4500.0 | nM | 5.35 |
| CHEMBL3908319 | — | IC50 | = | 4500.0 | nM | 5.35 |
| CHEMBL3893905 | — | IC50 | = | 6280.0 | nM | 5.20 |
| CHEMBL3893169 | — | IC50 | = | 6280.0 | nM | 5.20 |
| CHEMBL3900524 | — | IC50 | > | 30000.0 | nM | - |