Assay Detail
Binding
CHEMBL3888396
Review assay metadata, readout intent, target linkage, and publication context from the same page.
GTPγ[35S] Functional Assay: Functional activity was evaluated using GTPγ[35S] assay in CHO membrane extracts expressing recombinant hCB1 (human CB1) receptors or hCB2 (human CB2) receptors. The assay relies on the binding of GTPγ[35S], a radiolabeled nonhydrolyzable GTP analogue, to the G protein upon binding of an agonist of the G-protein-coupled receptor. In this system, agonists stimulate GTPγ[35S] binding whereas neutral antagonist have no effect and inverse agonists decrease GTPγ[35S] basal binding.
2
Total Activities
2
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Confidence | 8 — Homologous single protein target |
| Curated By | Autocuration |
Publication
Heterocyclic modulators of cannabinoid receptors
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| EC50 | 2 | 8.05 | 8.05 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL77520 | — | — | 1 | 8.05 |
| CHEMBL495528 | — | — | 1 | - |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL77520 | — | EC50 | = | 9.0 | nM | 8.05 |
| CHEMBL495528 | — | EC50 | > | 10000.0 | nM | - |