Assay Detail
Binding
CHEMBL3888858
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition Assay: Reader: Synergy HT program: tyrosinase 280-490 kinetics: kinetics over 45 minutes, reading at t=10 minutes, Tests in transparent 96-well plates, Phosphate buffer (pH 6.8), Enzyme: mushroom tyrosinase (T-3824, Sigma), Substrate: L-tyrosine (T-3754, Sigma), Positive control: Kojic acid (KA) (60890, Fluka) (reference inhibitor). Reference Molecules for the Test: Kojic acid: 9 μM<IC50<20 μM (PPO), 3 &muM<IC50<7 μM (MPMO); Vitamin C: 20 μM<IC50<40 μM (PPO); Reduced glutathione: 55% inhibition at 25 μM (PPO), IC50=1-2 μM (MPMO); Hydroquinone: IC50=3-4 μM (MPMO); Arbutin: 57% inhibition at 88 μM (MPMO).
11
Total Activities
11
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Confidence | 8 — Homologous single protein target |
| Curated By | Autocuration |
Publication
Heterocyclic resorcinol derivatives, preparation of same and cosmetic uses thereof
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 11 | 6.00 | 7.10 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL3965042 | — | — | 1 | 7.10 |
| CHEMBL3901907 | — | — | 1 | 7.05 |
| CHEMBL3961037 | — | — | 1 | 7.00 |
| CHEMBL3894834 | — | — | 1 | 6.70 |
| CHEMBL3921984 | — | — | 1 | 6.40 |
| CHEMBL450195 | RUCINOL | — | 1 | 6.00 |
| CHEMBL3985209 | — | — | 1 | 5.82 |
| CHEMBL3983018 | — | — | 1 | 5.70 |
| CHEMBL3895559 | — | — | 1 | 5.31 |
| CHEMBL287556 | KOJIC ACID | — | 1 | 4.82 |
| CHEMBL4109994 | — | — | 1 | 4.05 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL3965042 | — | IC50 | = | 80.0 | nM | 7.10 |
| CHEMBL3901907 | — | IC50 | = | 90.0 | nM | 7.05 |
| CHEMBL3961037 | — | IC50 | = | 100.0 | nM | 7.00 |
| CHEMBL3894834 | — | IC50 | = | 200.0 | nM | 6.70 |
| CHEMBL3921984 | — | IC50 | = | 400.0 | nM | 6.40 |
| CHEMBL450195 | RUCINOL | IC50 | = | 1000.0 | nM | 6.00 |
| CHEMBL3985209 | — | IC50 | = | 1500.0 | nM | 5.82 |
| CHEMBL3983018 | — | IC50 | = | 2000.0 | nM | 5.70 |
| CHEMBL3895559 | — | IC50 | = | 4900.0 | nM | 5.31 |
| CHEMBL287556 | KOJIC ACID | IC50 | = | 15000.0 | nM | 4.82 |
| CHEMBL4109994 | — | IC50 | = | 90000.0 | nM | 4.05 |