Assay Detail
Binding
CHEMBL3888930
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition Assay: The hERG inhibition study aims at quantifying the in vitro effects of compounds of the invention on the potassium-selective IKr current generated in normoxic conditions in stably transfected HEK 293 cells with the human ether-a-go-go-related gene (hERG). Whole-cell currents (acquisition by manual patch-clamp) elicited during a voltage pulse were recorded in baseline conditions and following application of tested compounds (5 minutes of exposure). The concentrations of tested compounds (0.3 μM; 3 μM; 10 μM; 30 μM) reflect a range believed to exceed the concentrations at expected efficacy doses in preclinical models. The pulses protocol applied is described as follow: the holding potential (every 3 seconds) was stepped from −80 mV to a maximum value of +40 mV, starting with −40 mV, in eight increments of +10 mV, for a period of 1 second. The membrane potential was then returned to −55 mV, after each of these incremented steps, for 1 second and finally repolarized to −80 mV for 1 second.
23
Total Activities
23
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Cell Type | Sf9 |
| Confidence | 8 — Homologous single protein target |
| Curated By | Autocuration |
Target
Voltage-gated inwardly rectifying potassium channel KCNH2 (CHEMBL240) ↗| Type | SINGLE PROTEIN |
| Organism | Homo sapiens |
Publication
Substituted [1,2,4]triazolo[4,3-a]pyrazines as selective NK-3 receptor antagonists
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 23 | 4.24 | 4.50 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL4108623 | — | — | 1 | 4.50 |
| CHEMBL4113741 | — | — | 1 | 4.50 |
| CHEMBL4108583 | — | — | 1 | 4.38 |
| CHEMBL4114280 | — | — | 1 | 4.35 |
| CHEMBL4109584 | — | — | 1 | 4.35 |
| CHEMBL4112585 | — | — | 1 | 4.34 |
| CHEMBL3608741 | — | — | 1 | 4.30 |
| CHEMBL3608740 | — | — | 1 | 4.30 |
| CHEMBL4107016 | — | — | 1 | 4.23 |
| CHEMBL4110242 | — | — | 1 | 4.21 |
| CHEMBL3608687 | — | — | 1 | 4.18 |
| CHEMBL4106866 | — | — | 1 | 4.16 |
| CHEMBL3608680 | FEZOLINETANT | 4.0 | 1 | 4.16 |
| CHEMBL4115594 | — | — | 1 | 4.16 |
| CHEMBL4115295 | — | — | 1 | 4.16 |
| CHEMBL4112037 | — | — | 1 | 4.16 |
| CHEMBL4113428 | — | — | 1 | 4.16 |
| CHEMBL4110286 | — | — | 1 | 4.16 |
| CHEMBL4110770 | — | — | 1 | 4.16 |
| CHEMBL4110224 | — | — | 1 | 4.16 |
| CHEMBL4112608 | — | — | 1 | 4.16 |
| CHEMBL4114258 | — | — | 1 | 4.16 |
| CHEMBL4111714 | — | — | 1 | 4.16 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL4108623 | — | IC50 | = | 32000.0 | nM | 4.50 |
| CHEMBL4113741 | — | IC50 | = | 32000.0 | nM | 4.50 |
| CHEMBL4108583 | — | IC50 | = | 42000.0 | nM | 4.38 |
| CHEMBL4114280 | — | IC50 | = | 45000.0 | nM | 4.35 |
| CHEMBL4109584 | — | IC50 | = | 45000.0 | nM | 4.35 |
| CHEMBL4112585 | — | IC50 | = | 46000.0 | nM | 4.34 |
| CHEMBL3608741 | — | IC50 | = | 50000.0 | nM | 4.30 |
| CHEMBL3608740 | — | IC50 | = | 50000.0 | nM | 4.30 |
| CHEMBL4107016 | — | IC50 | = | 59000.0 | nM | 4.23 |
| CHEMBL4110242 | — | IC50 | = | 61000.0 | nM | 4.21 |
| CHEMBL3608687 | — | IC50 | = | 66000.0 | nM | 4.18 |
| CHEMBL4106866 | — | IC50 | = | 70000.0 | nM | 4.16 |
| CHEMBL3608680 | FEZOLINETANT | IC50 | = | 70000.0 | nM | 4.16 |
| CHEMBL4115594 | — | IC50 | = | 70000.0 | nM | 4.16 |
| CHEMBL4115295 | — | IC50 | = | 70000.0 | nM | 4.16 |
| CHEMBL4112037 | — | IC50 | = | 70000.0 | nM | 4.16 |
| CHEMBL4113428 | — | IC50 | = | 70000.0 | nM | 4.16 |
| CHEMBL4110286 | — | IC50 | = | 70000.0 | nM | 4.16 |
| CHEMBL4110770 | — | IC50 | = | 70000.0 | nM | 4.16 |
| CHEMBL4110224 | — | IC50 | = | 70000.0 | nM | 4.16 |
| CHEMBL4112608 | — | IC50 | = | 70000.0 | nM | 4.16 |
| CHEMBL4114258 | — | IC50 | = | 70000.0 | nM | 4.16 |
| CHEMBL4111714 | — | IC50 | = | 70000.0 | nM | 4.16 |