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Assay Detail

CHEMBL3889237

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Electrophysiological Assay (In vitro Assay): Patch voltage clamp electrophysiology allows for the direct measurement and quantification of block of voltage-gated sodium channels (Nav's), and allows the determination of the time- and voltage-dependence of block which has been interpreted as differential binding to the resting, open, and inactivated states of the sodium channel (Hille, B., Journal of General Physiology (1977), 69: 497-515).The following patch voltage clamp electrophysiology studies were performed on representative compounds of the invention using human embryonic kidney cells (HEK), permanently transfected with an expression vector containing the full-length cDNA coding for the desired human sodium channel α-subunit, grown in culture media containing 10% FBS, 1% PSG, and 0.5 mg/mL G418 at 37° C. with 5% CO2. HEK cells used for the electrophysiology (EP) recordings had a passage number of less than 40 for all studies and were used within three days from the time of plating. Nav1.7 and Nav1.5 cDNAs (NM_00297
37
Total Activities
37
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Cell Type HEK293
Confidence 8 — Homologous single protein target
Curated By Autocuration

Target

Sodium channel protein type 9 subunit alpha (CHEMBL4296)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Biaryl ether sulfonamides and their use as therapeutic agents
(2016)

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 37 8.21 9.70

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3955386 1 9.70
CHEMBL3965272 1 9.52
CHEMBL3617063 1 9.52
CHEMBL3806065 1 9.30
CHEMBL3957974 1 9.30
CHEMBL3341983 1 9.22
CHEMBL3910564 1 9.15
CHEMBL3925113 1 9.10
CHEMBL3906826 1 9.00
CHEMBL3963109 1 9.00
CHEMBL3907762 1 8.92
CHEMBL3928901 1 8.85
CHEMBL3912540 1 8.85
CHEMBL3805973 1 8.77
CHEMBL3805722 1 8.77
CHEMBL3805837 1 8.77
CHEMBL3950598 1 8.74
CHEMBL3805201 1 8.52
CHEMBL3969795 1 8.46
CHEMBL3805347 1 8.44
CHEMBL3804898 1 8.36
CHEMBL3805897 1 8.28
CHEMBL3952736 1 8.13
CHEMBL3978280 1 8.10
CHEMBL3805563 1 8.06
CHEMBL3934922 1 7.97
CHEMBL3805265 1 7.87
CHEMBL3958847 1 7.76
CHEMBL3949421 1 7.75
CHEMBL3955084 1 7.63

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3955386 IC50 = 0.2 nM 9.70
CHEMBL3965272 IC50 = 0.3 nM 9.52
CHEMBL3617063 IC50 = 0.3 nM 9.52
CHEMBL3806065 IC50 = 0.5 nM 9.30
CHEMBL3957974 IC50 = 0.5 nM 9.30
CHEMBL3341983 IC50 = 0.6 nM 9.22
CHEMBL3910564 IC50 = 0.7 nM 9.15
CHEMBL3925113 IC50 = 0.8 nM 9.10
CHEMBL3906826 IC50 = 1.0 nM 9.00
CHEMBL3963109 IC50 = 1.0 nM 9.00
CHEMBL3907762 IC50 = 1.2 nM 8.92
CHEMBL3928901 IC50 = 1.4 nM 8.85
CHEMBL3912540 IC50 = 1.4 nM 8.85
CHEMBL3805837 IC50 = 1.7 nM 8.77
CHEMBL3805722 IC50 = 1.7 nM 8.77
CHEMBL3805973 IC50 = 1.7 nM 8.77
CHEMBL3950598 IC50 = 1.8 nM 8.74
CHEMBL3805201 IC50 = 3.0 nM 8.52
CHEMBL3969795 IC50 = 3.5 nM 8.46
CHEMBL3805347 IC50 = 3.6 nM 8.44
CHEMBL3804898 IC50 = 4.4 nM 8.36
CHEMBL3805897 IC50 = 5.3 nM 8.28
CHEMBL3952736 IC50 = 7.4 nM 8.13
CHEMBL3978280 IC50 = 8.0 nM 8.10
CHEMBL3805563 IC50 = 8.7 nM 8.06
CHEMBL3934922 IC50 = 10.8 nM 7.97
CHEMBL3805265 IC50 = 13.4 nM 7.87
CHEMBL3958847 IC50 = 17.4 nM 7.76
CHEMBL3949421 IC50 = 17.9 nM 7.75
CHEMBL3955084 IC50 = 23.2 nM 7.63
CHEMBL3805138 IC50 = 51.0 nM 7.29
CHEMBL3966575 IC50 = 67.8 nM 7.17
CHEMBL3805199 IC50 = 222.0 nM 6.65
CHEMBL3963256 IC50 = 674.6 nM 6.17
CHEMBL3805875 IC50 = 2308.8 nM 5.64
CHEMBL3983385 IC50 = 2375.6 nM 5.62
CHEMBL3893190 IC50 = 3378.2 nM 5.47