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Assay Detail

CHEMBL3997545

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of full length recombinant human LOXL2 expressed in CHO cells spiked into human whole blood assessed as reduction in H2O2 production using DAP as substrate preincubated for 2 followed by substrate addition measured every 90 secs for 45 mins by Amplex red dye based fluorescence assay
2
Total Activities
2
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Lysyl oxidase homolog 2 (CHEMBL3714029)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Small Molecule Lysyl Oxidase-like 2 (LOXL2) Inhibitors: The Identification of an Inhibitor Selective for LOXL2 over LOX.
Hutchinson JH, Rowbottom MW, Lonergan D, Darlington J, Prodanovich P, King CD, Evans JF, Bain G.
ACS Med Chem Lett (2017) 8 : 423 -427
PubMed 28435530 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 2 6.12 6.40

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1618272 1 6.40
CHEMBL4068784 1 5.84

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1618272 IC50 = 396.0 nM 6.40
CHEMBL4068784 IC50 = 1450.0 nM 5.84