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Assay Detail

CHEMBL3999296

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human RIP1 (1 to 375 residues) expressed in baculovirus infected insect cells preincubated for 1 hr followed by ATP addition measured after 5 hrs by ADP-Glo luminescence assay
20
Total Activities
20
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Receptor-interacting serine/threonine-protein kinase 1 (CHEMBL5464)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of a First-in-Class Receptor Interacting Protein 1 (RIP1) Kinase Specific Clinical Candidate (GSK2982772) for the Treatment of Inflammatory Diseases.
Harris PA, Berger SB, Jeong JU, Nagilla R, Bandyopadhyay D, Campobasso N, Capriotti CA, Cox JA, Dare L, Dong X, Eidam PM, Finger JN, Hoffman SJ, Kang J, Kasparcova V, King BW, Lehr R, Lan Y, Leister LK, Lich JD, MacDonald TT, Miller NA, Ouellette MT, Pao CS, Rahman A, Reilly MA, Rendina AR, Rivera EJ, Schaeffer MC, Sehon CA, Singhaus RR, Sun HH, Swift BA, Totoritis RD, Vossenkämper A, Ward P, Wisnoski DD, Zhang D, Marquis RW, Gough PJ, Bertin J.
J Med Chem (2017) 60 : 1247 -1261
PubMed 28151659 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 20 9.25 10.30

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4067372 1 10.30
CHEMBL3785745 1 10.20
CHEMBL4093220 1 10.10
CHEMBL3786997 1 9.70
CHEMBL4068954 1 9.49
CHEMBL4096224 1 9.49
CHEMBL4060644 1 9.40
CHEMBL4069318 1 9.40
CHEMBL4099004 1 9.30
CHEMBL4089558 1 9.30
CHEMBL4065561 1 9.20
CHEMBL4081685 1 9.20
CHEMBL4088857 1 9.10
CHEMBL3786078 1 9.10
CHEMBL4099698 1 9.00
CHEMBL4071864 GSK2982772 2.0 1 9.00
CHEMBL4080978 1 9.00
CHEMBL4071080 1 8.70
CHEMBL4069959 1 8.30
CHEMBL3786293 1 7.80

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4067372 IC50 = 0.05 nM 10.30
CHEMBL3785745 IC50 = 0.063 nM 10.20
CHEMBL4093220 IC50 = 0.079 nM 10.10
CHEMBL3786997 IC50 = 0.2 nM 9.70
CHEMBL4068954 IC50 = 0.32 nM 9.49
CHEMBL4096224 IC50 = 0.32 nM 9.49
CHEMBL4060644 IC50 = 0.4 nM 9.40
CHEMBL4069318 IC50 = 0.4 nM 9.40
CHEMBL4099004 IC50 = 0.5 nM 9.30
CHEMBL4089558 IC50 = 0.5 nM 9.30
CHEMBL4065561 IC50 = 0.63 nM 9.20
CHEMBL4081685 IC50 = 0.63 nM 9.20
CHEMBL4088857 IC50 = 0.79 nM 9.10
CHEMBL3786078 IC50 = 0.79 nM 9.10
CHEMBL4099698 IC50 = 1.0 nM 9.00
CHEMBL4071864 GSK2982772 IC50 = 1.0 nM 9.00
CHEMBL4080978 IC50 = 1.0 nM 9.00
CHEMBL4071080 IC50 = 2.0 nM 8.70
CHEMBL4069959 IC50 = 5.0 nM 8.30
CHEMBL3786293 IC50 = 16.0 nM 7.80