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Assay Detail

CHEMBL3999828

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of Proteus mirabilis 543 urease at pH 5.5 assessed as reduction in ammonia production using urea as substrate preincubated for 120 mins followed by substrate addition measured after 15 mins by NaOH-hypochlorite method
5
Total Activities
5
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Proteus mirabilis
Confidence 0 — Uncurated / Unknown
Curated By Autocuration

Target

Unchecked (CHEMBL612545)
Type UNCHECKED

Publication

Potent covalent inhibitors of bacterial urease identified by activity-reactivity profiling.
Macegoniuk K, Kowalczyk R, Rudzińska A, Psurski M, Wietrzyk J, Berlicki Ł.
Bioorg Med Chem Lett (2017) 27 : 1346 -1350
PubMed 28236590 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 5 4.09 4.18

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL503160 FUMARIC ACID 3.0 1 4.18
CHEMBL1874364 1 4.05
CHEMBL734 ACETOHYDROXAMIC ACID 4.0 1 4.04
CHEMBL318196 ETHYL CINNAMATE 1 -
CHEMBL27246 CINNAMIC ACID 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL503160 FUMARIC ACID IC50 = 65500.0 nM 4.18
CHEMBL1874364 IC50 = 88600.0 nM 4.05
CHEMBL734 ACETOHYDROXAMIC ACID IC50 = 91400.0 nM 4.04
CHEMBL318196 ETHYL CINNAMATE IC50 - -
CHEMBL27246 CINNAMIC ACID IC50 = 125000.0 nM -