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Assay Detail

CHEMBL4000266

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Agonist activity at CB1 receptor in rat brain membranes assessed as reduction in forskolin-stimulated cAMP level measured from 8 data points after 30 mins
3
Total Activities
3
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Rattus norvegicus
Tissue Brain
Subcellular Membrane
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Cannabinoid receptor 1 (CHEMBL3571)
Type SINGLE PROTEIN
Organism Rattus norvegicus

Publication

Probing the carboxyester side chain in controlled deactivation (-)-δ(8)-tetrahydrocannabinols.
Nikas SP, Sharma R, Paronis CA, Kulkarni S, Thakur GA, Hurst D, Wood JT, Gifford RS, Rajarshi G, Liu Y, Raghav JG, Guo JJ, Järbe TU, Reggio PH, Bergman J, Makriyannis A.
J Med Chem (2015) 58 : 665 -681
PubMed 25470070 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 3 9.25 9.40

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3104362 1 9.40
CHEMBL3104360 1 9.30
CHEMBL4071389 1 9.05

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3104362 EC50 = 0.4 nM 9.40
CHEMBL3104360 EC50 = 0.5 nM 9.30
CHEMBL4071389 EC50 = 0.9 nM 9.05