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Assay Detail

CHEMBL4003695

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of N-terminal His6-tagged full-length recombinant human NEK2 expressed in baculovirus infected sf21 cells using biotin labelled-STK-3 substrate measured after 60 mins by HTRF assay
26
Total Activities
26
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Serine/threonine-protein kinase Nek2 (CHEMBL3835)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Structure-based design and synthesis of imidazo[1,2-a]pyridine derivatives as novel and potent Nek2 inhibitors with in vitro and in vivo antitumor activities.
Xi JB, Fang YF, Frett B, Zhu ML, Zhu T, Kong YN, Guan FJ, Zhao Y, Zhang XW, Li HY, Ma ML, Hu W.
Eur J Med Chem (2017) 126 : 1083 -1106
PubMed 28039836 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 26 6.06 9.00

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4062453 1 9.00
CHEMBL4063211 1 8.52
CHEMBL4091089 1 8.15
CHEMBL4087758 1 7.06
CHEMBL4105643 1 6.41
CHEMBL4101298 1 6.32
CHEMBL4062949 1 6.23
CHEMBL4065063 1 6.09
CHEMBL4102393 1 6.08
CHEMBL4083300 1 6.03
CHEMBL4076354 1 5.98
CHEMBL4082341 1 5.92
CHEMBL4098835 1 5.89
CHEMBL4084616 1 5.80
CHEMBL4064022 1 5.66
CHEMBL4076233 1 5.62
CHEMBL4060438 1 5.49
CHEMBL4086508 1 5.00
CHEMBL4083100 1 5.00
CHEMBL4071842 1 5.00
CHEMBL4073648 1 4.95
CHEMBL4102006 1 4.71
CHEMBL4091785 1 4.37
CHEMBL4061228 1 -
CHEMBL4094275 1 -
CHEMBL4091915 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4062453 IC50 = 1.0 nM 9.00
CHEMBL4063211 IC50 = 3.0 nM 8.52
CHEMBL4091089 IC50 = 7.0 nM 8.15
CHEMBL4087758 IC50 = 87.0 nM 7.06
CHEMBL4105643 IC50 = 386.0 nM 6.41
CHEMBL4101298 IC50 = 478.0 nM 6.32
CHEMBL4062949 IC50 = 591.0 nM 6.23
CHEMBL4065063 IC50 = 808.0 nM 6.09
CHEMBL4102393 IC50 = 838.0 nM 6.08
CHEMBL4083300 IC50 = 927.0 nM 6.03
CHEMBL4076354 IC50 = 1037.0 nM 5.98
CHEMBL4082341 IC50 = 1190.0 nM 5.92
CHEMBL4098835 IC50 = 1300.0 nM 5.89
CHEMBL4084616 IC50 = 1600.0 nM 5.80
CHEMBL4064022 IC50 = 2170.0 nM 5.66
CHEMBL4076233 IC50 = 2400.0 nM 5.62
CHEMBL4060438 IC50 = 3210.0 nM 5.49
CHEMBL4086508 IC50 = 10030.0 nM 5.00
CHEMBL4083100 IC50 = 10030.0 nM 5.00
CHEMBL4071842 IC50 = 10000.0 nM 5.00
CHEMBL4073648 IC50 = 11190.0 nM 4.95
CHEMBL4102006 IC50 = 19280.0 nM 4.71
CHEMBL4091785 IC50 = 42600.0 nM 4.37
CHEMBL4061228 IC50 = 197000.0 nM -
CHEMBL4094275 IC50 > 10000.0 nM -
CHEMBL4091915 IC50 > 200000.0 nM -