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Assay Detail

CHEMBL4005264

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Functional
Agonist activity at recombinant human IP receptor expressed in CHO-K1 cells assessed as increase in intracellular cAMP level after 1 hr incubation by HTRF method
26
Total Activities
26
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Prostacyclin receptor (CHEMBL1995)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of 2-(((1r,4r)-4-(((4-Chlorophenyl)(phenyl)carbamoyl)oxy)methyl)cyclohexyl)methoxy)acetate (Ralinepag): An Orally Active Prostacyclin Receptor Agonist for the Treatment of Pulmonary Arterial Hypertension.
Tran TA, Kramer B, Shin YJ, Vallar P, Boatman PD, Zou N, Sage CR, Gharbaoui T, Krishnan A, Pal B, Shakya SR, Garrido Montalban A, Adams JW, Ramirez J, Behan DP, Shifrina A, Blackburn A, Leakakos T, Shi Y, Morgan M, Sadeque A, Chen W, Unett DJ, Gaidarov I, Chen X, Chang S, Shu HH, Tung SF, Semple G.
J Med Chem (2017) 60 : 913 -927
PubMed 28072531 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 26 7.66 8.62

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL494 ILOPROST 4.0 1 8.62
CHEMBL3893346 1 8.48
CHEMBL3926078 1 8.47
CHEMBL3935924 1 8.41
CHEMBL3932106 1 8.28
CHEMBL238804 SELEXIPAG 4.0 1 8.22
CHEMBL3975122 1 8.18
CHEMBL3983767 1 8.11
CHEMBL3301604 RALINEPAG 3.0 1 8.07
CHEMBL3933704 1 8.06
CHEMBL3981509 1 8.00
CHEMBL3928729 1 7.92
CHEMBL3914174 1 7.73
CHEMBL3966307 1 7.72
CHEMBL3952237 1 7.68
CHEMBL239226 LASELIPAG 2.0 1 7.66
CHEMBL3943791 1 7.64
CHEMBL3896580 1 7.60
CHEMBL3890685 1 7.42
CHEMBL3952439 1 7.39
CHEMBL3919269 1 7.34
CHEMBL3900038 1 7.27
CHEMBL3922000 1 7.21
CHEMBL3917503 1 6.03
CHEMBL4061744 1 5.98
CHEMBL3930990 1 5.79

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL494 ILOPROST EC50 = 2.4 nM 8.62
CHEMBL3893346 EC50 = 3.3 nM 8.48
CHEMBL3926078 EC50 = 3.4 nM 8.47
CHEMBL3935924 EC50 = 3.9 nM 8.41
CHEMBL3932106 EC50 = 5.2 nM 8.28
CHEMBL238804 SELEXIPAG EC50 = 6.0 nM 8.22
CHEMBL3975122 EC50 = 6.6 nM 8.18
CHEMBL3983767 EC50 = 7.8 nM 8.11
CHEMBL3301604 RALINEPAG EC50 = 8.5 nM 8.07
CHEMBL3933704 EC50 = 8.7 nM 8.06
CHEMBL3981509 EC50 = 10.0 nM 8.00
CHEMBL3928729 EC50 = 12.0 nM 7.92
CHEMBL3914174 EC50 = 18.5 nM 7.73
CHEMBL3966307 EC50 = 19.0 nM 7.72
CHEMBL3952237 EC50 = 21.0 nM 7.68
CHEMBL239226 LASELIPAG EC50 = 22.0 nM 7.66
CHEMBL3943791 EC50 = 23.0 nM 7.64
CHEMBL3896580 EC50 = 25.0 nM 7.60
CHEMBL3890685 EC50 = 38.0 nM 7.42
CHEMBL3952439 EC50 = 41.0 nM 7.39
CHEMBL3919269 EC50 = 46.0 nM 7.34
CHEMBL3900038 EC50 = 54.0 nM 7.27
CHEMBL3922000 EC50 = 62.0 nM 7.21
CHEMBL3917503 EC50 = 930.0 nM 6.03
CHEMBL4061744 EC50 = 1050.0 nM 5.98
CHEMBL3930990 EC50 = 1630.0 nM 5.79