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Assay Detail

CHEMBL4005280

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of [3H]-iloprost from recombinant human IP receptor expressed in CHO-K1 cell membranes incubated for 1 hr by top count scintillation counting method
6
Total Activities
6
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Prostacyclin receptor (CHEMBL1995)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of 2-(((1r,4r)-4-(((4-Chlorophenyl)(phenyl)carbamoyl)oxy)methyl)cyclohexyl)methoxy)acetate (Ralinepag): An Orally Active Prostacyclin Receptor Agonist for the Treatment of Pulmonary Arterial Hypertension.
Tran TA, Kramer B, Shin YJ, Vallar P, Boatman PD, Zou N, Sage CR, Gharbaoui T, Krishnan A, Pal B, Shakya SR, Garrido Montalban A, Adams JW, Ramirez J, Behan DP, Shifrina A, Blackburn A, Leakakos T, Shi Y, Morgan M, Sadeque A, Chen W, Unett DJ, Gaidarov I, Chen X, Chang S, Shu HH, Tung SF, Semple G.
J Med Chem (2017) 60 : 913 -927
PubMed 28072531 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 6 7.89 8.52

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3301604 RALINEPAG 3.0 1 8.52
CHEMBL494 ILOPROST 4.0 1 8.49
CHEMBL3933704 1 8.15
CHEMBL3917503 1 6.40
CHEMBL3896580 1 -
CHEMBL4061744 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3301604 RALINEPAG Ki = 3.0 nM 8.52
CHEMBL494 ILOPROST Ki = 3.2 nM 8.49
CHEMBL3933704 Ki = 7.0 nM 8.15
CHEMBL3917503 Ki = 400.0 nM 6.40
CHEMBL3896580 Ki - -
CHEMBL4061744 Ki - -